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Biosynthetic Machinery of Diterpene Pleuromutilin Isolated from Basidiomycete Fungi

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Title: Biosynthetic Machinery of Diterpene Pleuromutilin Isolated from Basidiomycete Fungi
Authors: Yamane, Momoka Browse this author
Minami, Atsushi Browse this author →KAKEN DB
Liu, Chengwei Browse this author
Ozaki, Taro Browse this author
Takeuchi, Ichiro Browse this author
Tsukagoshi, Tae Browse this author
Tokiwano, Tetsuo Browse this author
Gomi, Katsuya Browse this author
Oikawa, Hideaki Browse this author →KAKEN DB
Keywords: biosynthesis
gene expression
natural products
Issue Date: 5-Dec-2017
Publisher: Wiley-Blackwell
Journal Title: Chembiochem
Volume: 18
Issue: 23
Start Page: 2317
End Page: 2322
Publisher DOI: 10.1002/cbic.201700434
PMID: 28924980
Abstract: The diterpene pleuromutilin is a ribosome-targeting antibiotic isolated from basidiomycete fungi, such as Clitopilus pseudo-pinsitus. The functional characterization of all biosynthetic enzymes involved in pleuromutilin biosynthesis is reported and a biosynthetic pathway proposed. In vitro enzymatic reactions and mutational analysis revealed that a labdane-related diterpene synthase, Ple3, catalyzed two rounds of cyclization from geranylgeranyl diphosphate to premutilin possessing a characteristic 5-6-8-tricyclic carbon skeleton. Biotransformation experiments utilizing Aspergillus oryzae transformants possessing modification enzyme genes allowed the biosynthetic pathway from premutilin to pleuromutilin to be proposed. The present study sets the stage for the enzymatic synthesis of natural products isolated from basidiomycete fungi, which are a prolific source of structurally diverse and biologically active terpenoids.
Rights: This is the peer-reviewed version of the following article: M. Yamane, A. Minami, C. Liu, T. Ozaki, I. Takeuchi, T. Tsukagoshi, T. Tokiwano, K. Gomi, H. Oikawa, ChemBioChem 2017, 18, 2317, which has been published in final form at DOI: 10.1002/cbic.201700434. This article may be used for non-commercial purposes in accordance with Wiley-VCH Terms and Conditions for Self-Archiving.
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 及川 英秋

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