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Biosynthetic Machinery of Diterpene Pleuromutilin Isolated from Basidiomycete Fungi

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タイトル: Biosynthetic Machinery of Diterpene Pleuromutilin Isolated from Basidiomycete Fungi
著者: Yamane, Momoka 著作を一覧する
Minami, Atsushi 著作を一覧する
Liu, Chengwei 著作を一覧する
Ozaki, Taro 著作を一覧する
Takeuchi, Ichiro 著作を一覧する
Tsukagoshi, Tae 著作を一覧する
Tokiwano, Tetsuo 著作を一覧する
Gomi, Katsuya 著作を一覧する
Oikawa, Hideaki 著作を一覧する
キーワード: biosynthesis
gene expression
natural products
発行日: 2017年12月 5日
出版者: Wiley-Blackwell
誌名: Chembiochem
巻: 18
号: 23
開始ページ: 2317
終了ページ: 2322
出版社 DOI: 10.1002/cbic.201700434
PMID: 28924980
抄録: The diterpene pleuromutilin is a ribosome-targeting antibiotic isolated from basidiomycete fungi, such as Clitopilus pseudo-pinsitus. The functional characterization of all biosynthetic enzymes involved in pleuromutilin biosynthesis is reported and a biosynthetic pathway proposed. In vitro enzymatic reactions and mutational analysis revealed that a labdane-related diterpene synthase, Ple3, catalyzed two rounds of cyclization from geranylgeranyl diphosphate to premutilin possessing a characteristic 5-6-8-tricyclic carbon skeleton. Biotransformation experiments utilizing Aspergillus oryzae transformants possessing modification enzyme genes allowed the biosynthetic pathway from premutilin to pleuromutilin to be proposed. The present study sets the stage for the enzymatic synthesis of natural products isolated from basidiomycete fungi, which are a prolific source of structurally diverse and biologically active terpenoids.
Rights: This is the peer-reviewed version of the following article: M. Yamane, A. Minami, C. Liu, T. Ozaki, I. Takeuchi, T. Tsukagoshi, T. Tokiwano, K. Gomi, H. Oikawa, ChemBioChem 2017, 18, 2317, which has been published in final form at DOI: 10.1002/cbic.201700434. This article may be used for non-commercial purposes in accordance with Wiley-VCH Terms and Conditions for Self-Archiving.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 及川 英秋


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