| Title: | An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair : A preclinical proof-of-concept study |
| Authors: | Tsujimoto, Takeru Browse this author |
| Sudo, Hideki Browse this author →KAKEN DB |
| Todoh, Masahiro Browse this author →KAKEN DB |
| Yamada, Katsuhisa Browse this author |
| Iwasaki, Koji Browse this author |
| Ohnishi, Takashi Browse this author |
| Hirohama, Naoki Browse this author |
| Nonoyama, Takayuki Browse this author |
| Ukeba, Daisuke Browse this author |
| Ura, Katsuro Browse this author |
| Ito, Yoichi M. Browse this author →KAKEN DB |
| Iwasaki, Norimasa Browse this author →KAKEN DB |
| Keywords: | intervertebral disc |
| Biomaterials |
| Biomechanics |
| Tissue engineering |
| Issue Date: | Nov-2018 |
| Publisher: | Elsevier |
| Journal Title: | EBioMedicine |
| Volume: | 37 |
| Start Page: | 521 |
| End Page: | 534 |
| Publisher DOI: | 10.1016/j.ebiom.2018.10.055 |
| Abstract: | Background: The current surgical procedure of choice for lumbar intervertebral disc (IVD) herniation is discectomy. However, defects within IVD produced upon discectomy may impair tissue healing and predispose patients to subsequent IVD degeneration. This study aimed to investigate whether the use of an acellular bioresorbable ultra-purified alginate (UPAL) gel implantation system is safe and effective as a reparative therapeutic strategy after lumbar discectomy. Methods: Human IVD cells were cultured in a three-dimensional system in UPAL gel. In addition, lumbar spines of sheep were used for mechanical analysis. Finally, the gel was implanted into IVD after discectomy in rabbits and sheep in vivo. Findings: The UPAL gel was biocompatible with human IVD cells and promoted extracellular matrix production after discectomy, demonstrating sufficient biomechanical characteristics without material protrusion. Interpretation: The present results indicate the safety and efficacy of UPAL gels in a large animal model and suggest that these gels represent a novel therapeutic strategy after discectomy in cases of lumbar IVD herniation. Fund: Grant-in-Aid for the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd. |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/72940 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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