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An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair : A preclinical proof-of-concept study

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/72940

Title: An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair : A preclinical proof-of-concept study
Authors: Tsujimoto, Takeru Browse this author
Sudo, Hideki Browse this author →KAKEN DB
Todoh, Masahiro Browse this author →KAKEN DB
Yamada, Katsuhisa Browse this author
Iwasaki, Koji Browse this author
Ohnishi, Takashi Browse this author
Hirohama, Naoki Browse this author
Nonoyama, Takayuki Browse this author
Ukeba, Daisuke Browse this author
Ura, Katsuro Browse this author
Ito, Yoichi M. Browse this author →KAKEN DB
Iwasaki, Norimasa Browse this author →KAKEN DB
Keywords: intervertebral disc
Biomaterials
Biomechanics
Tissue engineering
Issue Date: Nov-2018
Publisher: Elsevier
Journal Title: EBioMedicine
Volume: 37
Start Page: 521
End Page: 534
Publisher DOI: 10.1016/j.ebiom.2018.10.055
Abstract: Background: The current surgical procedure of choice for lumbar intervertebral disc (IVD) herniation is discectomy. However, defects within IVD produced upon discectomy may impair tissue healing and predispose patients to subsequent IVD degeneration. This study aimed to investigate whether the use of an acellular bioresorbable ultra-purified alginate (UPAL) gel implantation system is safe and effective as a reparative therapeutic strategy after lumbar discectomy. Methods: Human IVD cells were cultured in a three-dimensional system in UPAL gel. In addition, lumbar spines of sheep were used for mechanical analysis. Finally, the gel was implanted into IVD after discectomy in rabbits and sheep in vivo. Findings: The UPAL gel was biocompatible with human IVD cells and promoted extracellular matrix production after discectomy, demonstrating sufficient biomechanical characteristics without material protrusion. Interpretation: The present results indicate the safety and efficacy of UPAL gels in a large animal model and suggest that these gels represent a novel therapeutic strategy after discectomy in cases of lumbar IVD herniation. Fund: Grant-in-Aid for the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd.
Rights: http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/72940
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 須藤 英毅

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