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Interferon stimulation creates chromatin marks and establishes transcriptional memory

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Title: Interferon stimulation creates chromatin marks and establishes transcriptional memory
Authors: Kamada, Rui Browse this author
Yang, Wenjing Browse this author
Zhang, Yubo Browse this author
Patel, Mira C. Browse this author
Yang, Yanqin Browse this author
Ouda, Ryota Browse this author
Dey, Anup Browse this author
Wakabayashi, Yoshiyuki Browse this author
Sakaguchi, Kazuyasu Browse this author →KAKEN DB
Fujita, Takashi Browse this author
Tamura, Tomohiko Browse this author
Zhu, Jun Browse this author
Ozato, Keiko Browse this author
Keywords: memory
innate immunity
histone H3.3
Issue Date: 25-Sep-2018
Publisher: National Academy of Sciences
Journal Title: Proceedings of the National Academy of Sciences of the United States of America (PNAS)
Volume: 115
Issue: 39
Start Page: E9162
End Page: E9171
Publisher DOI: 10.1073/pnas.1720930115
Abstract: Epigenetic memory for signal-dependent transcription has remained elusive. So far, the concept of epigenetic memory has been largely limited to cell-autonomous, preprogrammed processes such as development and metabolism. Here we show that IFN beta stimulation creates transcriptional memory in fibroblasts, conferring faster and greater transcription upon restimulation. The memory was inherited through multiple cell divisions and led to improved antiviral protection. Of similar to 2,000 IFN beta-stimulated genes (ISGs), about half exhibited memory, which we define as memory ISGs. The rest, designated nonmemory ISGs, did not show memory. Surprisingly, mechanistic analysis showed that IFN memory was not due to enhanced IFN signaling or retention of transcription factors on the ISGs. We demonstrated that this memory was attributed to accelerated recruitment of RNA polymerase II and transcription/chromatin factors, which coincided with acquisition of the histone H3.3 and H3K36me3 chromatin marks on memory ISGs. Similar memory was observed in bone marrow macrophages after IFN. stimulation, suggesting that IFN stimulation modifies the shape of the innate immune response. Together, external signals can establish epigenetic memory in mammalian cells that imparts lasting adaptive performance upon various somatic cells.
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鎌田 瑠泉

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