| Title: | Interferon stimulation creates chromatin marks and establishes transcriptional memory |
| Authors: | Kamada, Rui Browse this author |
| Yang, Wenjing Browse this author |
| Zhang, Yubo Browse this author |
| Patel, Mira C. Browse this author |
| Yang, Yanqin Browse this author |
| Ouda, Ryota Browse this author |
| Dey, Anup Browse this author |
| Wakabayashi, Yoshiyuki Browse this author |
| Sakaguchi, Kazuyasu Browse this author →KAKEN DB |
| Fujita, Takashi Browse this author |
| Tamura, Tomohiko Browse this author |
| Zhu, Jun Browse this author |
| Ozato, Keiko Browse this author |
| Keywords: | memory |
| interferons |
| transcription |
| innate immunity |
| histone H3.3 |
| Issue Date: | 25-Sep-2018 |
| Publisher: | National Academy of Sciences |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America (PNAS) |
| Volume: | 115 |
| Issue: | 39 |
| Start Page: | E9162 |
| End Page: | E9171 |
| Publisher DOI: | 10.1073/pnas.1720930115 |
| Abstract: | Epigenetic memory for signal-dependent transcription has remained elusive. So far, the concept of epigenetic memory has been largely limited to cell-autonomous, preprogrammed processes such as development and metabolism. Here we show that IFN beta stimulation creates transcriptional memory in fibroblasts, conferring faster and greater transcription upon restimulation. The memory was inherited through multiple cell divisions and led to improved antiviral protection. Of similar to 2,000 IFN beta-stimulated genes (ISGs), about half exhibited memory, which we define as memory ISGs. The rest, designated nonmemory ISGs, did not show memory. Surprisingly, mechanistic analysis showed that IFN memory was not due to enhanced IFN signaling or retention of transcription factors on the ISGs. We demonstrated that this memory was attributed to accelerated recruitment of RNA polymerase II and transcription/chromatin factors, which coincided with acquisition of the histone H3.3 and H3K36me3 chromatin marks on memory ISGs. Similar memory was observed in bone marrow macrophages after IFN. stimulation, suggesting that IFN stimulation modifies the shape of the innate immune response. Together, external signals can establish epigenetic memory in mammalian cells that imparts lasting adaptive performance upon various somatic cells. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/73330 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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