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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
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Astaxanthin improves the developmental competence of invitro-grown oocytes and modifies the steroidogenesis of granulosa cells derived from bovine early antral follicles
| Title: | Astaxanthin improves the developmental competence of invitro-grown oocytes and modifies the steroidogenesis of granulosa cells derived from bovine early antral follicles |
| Authors: | Abdel-Ghani, M. A. Browse this author | | Yanagawa, Y. Browse this author | | Balboula, A. Z. Browse this author | | Sakaguchi, K. Browse this author | | Kanno, C. Browse this author | | Katagiri, S. Browse this author | | Takahashi, M. Browse this author →KAKEN DB | | Nagano, M. Browse this author |
| Keywords: | cathepsin B | | invitro growth | | reactive oxygen species generation |
| Issue Date: | Jan-2019 |
| Publisher: | CSIRO Publishing |
| Journal Title: | Reproduction, Fertility and Development |
| Volume: | 31 |
| Issue: | 2 |
| Start Page: | 272 |
| End Page: | 281 |
| Publisher DOI: | 10.1071/RD17527 |
| Abstract: | In this study we investigated the effect of astaxanthin (Ax), which exhibits strong antioxidant activity, during invitro growth (IVG) on the developmental competence of oocytes and steroidogenesis of granulosa cells derived from early antral follicles. Bovine oocyte-cumulus-granulosa complexes collected from early antral follicles were cultured for 12 days in the presence or absence (control) of 500 mu M Ax. The viability of oocytes and antrum formation in the granulosa cell layer during IVG culture were greater in the presence than absence of Ax (P<0.05). Regardless of Ax treatment, 17-oestradiol production increased during IVG culture; however, progesterone production was significantly lower in the presence than absence of Ax (P<0.05). Reactive oxygen species levels were lower in Ax-treated oocytes than in controls after IVG (P<0.05). Although nuclear maturation and cleavage rates did not differ between the Ax-treated and control groups, Ax treatment led to weaker cathepsin B activity in oocytes and better blastocyst rates than in controls (P<0.05). Accordingly, Ax treatment during IVG increased the total number of cells in blastocysts (P<0.05). These results indicate that Ax supplementation of IVG medium improves the quality of bovine oocytes due to its antioxidative effects on growing oocytes and its suppression of the luteinisation of granulosa cells. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/73394 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永野 昌志
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