HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >

Murine intestinal organoids resemble intestinal epithelium in their microRNA profiles

Files in This Item:
Ohsaka2017BBB_R2 -fig.pdf2.4 MBPDFView/Open
STabel1.xlsxSupplementary file34.33 kBMicrosoft Excel XMLView/Open
STable2.xlsxSupplementary file93.11 kBMicrosoft Excel XMLView/Open
Please use this identifier to cite or link to this item:

Title: Murine intestinal organoids resemble intestinal epithelium in their microRNA profiles
Authors: Ohsaka, Fumina Browse this author
Sonoyama, Kei Browse this author →KAKEN DB
Keywords: MicroRNA
intestinal epithelium
intestinal organoid
Issue Date: 8-May-2018
Publisher: Taylor & Francis
Journal Title: Bioscience biotechnology and biochemistry
Volume: 82
Issue: 9
Start Page: 1560
End Page: 1567
Publisher DOI: 10.1080/09168451.2018.1469397
PMID: 29739279
Abstract: Intestinal organoids were established as an ex vivo model of the intestinal epithelium. We investigated whether organoids resemble the intestinal epithelium in their microRNA (miRNA) profiles. Total RNA samples were obtained from crypt and villus fractions in murine intestine and from cultured organoids. Microarray analysis showed that organoids largely resembled intestinal epithelial cells in their miRNA profiles. In silico prediction followed by qRT-PCR suggested that six genes are regulated by corresponding miRNAs along the crypt-villus axis, suggesting miRNA regulation of epithelial cell renewal in the intestine. However, such expression patterns of miRNAs and their target mRNAs were not reproduced during organoids maturation. This might be due to lack of luminal factors and endocrine, nervous, and immune systems in organoids and different cell populations between in vivo epithelium and organoids. Nevertheless, we propose that intestinal organoids provide a useful in vitro model to investigate miRNA expression in intestinal epithelial cells.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 08 May 2018, available online:
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 園山 慶

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University