|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
| Title: | Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia |
| Authors: | Qiu, Yongjin Browse this author | | Kaneko, Chiho Browse this author | | Kajihara, Masahiro Browse this author →KAKEN DB | | Ngonda, Saasa Browse this author | | Simulundu, Edgar Browse this author | | Muleya, Walter Browse this author | | Thu, May June Browse this author | | Hang'ombe, Mudenda Bernard Browse this author | | Katakura, Ken Browse this author →KAKEN DB | | Takada, Ayato Browse this author →KAKEN DB | | Sawa, Hirofumi Browse this author →KAKEN DB | | Simuunza, Martin Browse this author | | Nakao, Ryo Browse this author →KAKEN DB |
| Keywords: | Anaplasmataceae | | Babesia | | Dog | | Hepatozoon | | Multiple PCR | | Zambia |
| Issue Date: | May-2018 |
| Publisher: | Elsevier |
| Journal Title: | Ticks and Tick-Borne Diseases |
| Volume: | 9 |
| Issue: | 4 |
| Start Page: | 988 |
| End Page: | 995 |
| Publisher DOI: | 10.1016/j.ttbdis.2018.03.025 |
| Abstract: | Tick-borne diseases (TBDs), including emerging and re-emerging infectious diseases, are important threats to human and animal health worldwide. Indeed, the number of reported human and animal infectious cases of novel TBD agents has increased in recent decades. However, TBDs tend to be neglected, especially in resourcelimited countries that often have limited diagnostic capacity. The aim of this molecular survey was to detect and characterise tick-borne pathogens (Babesia, Theileria, and Hepatozoon parasites and Anaplasmataceae bacteria) in domestic dogs in Zambia. In total, 247 canine peripheral blood samples were collected in Lusaka, Mazabuka, Monze, and Shangombo. Conventional PCR to detect the selected pathogens was performed using DNA extracted from canine blood. One hundred eleven samples were positive for protozoa and 5 were positive for Anaplasmataceae. Sequencing of thirty-five randomly selected protozoa-positive samples revealed the presence of Babesia rossi, Babesia vogeli, and Hepatozoon canis 18S rDNA. Based on these sequences, a multiplex PCR system was developed to yield PCR products with different amplicons, the size of which depended on the parasite species; thus, each species could be identified without the need for sequence analysis. Approximately 40% of dogs were positive for H. canis. In particular, the positive rate (75.2%) of H. canis infection was significantly higher in Shangombo than in other sampling sites. Multiplex PCR assay detected B. rossi and B. vogeli infections in five and seven dogs, respectively, indicating that this approach is useful for detecting parasites with low prevalence. Sequencing analysis of gltA and groEL genes of Anaplasmataceae revealed that two and one dogs in Lusaka were infected with Anaplasma platys and Ehrlichia canis, respectively. The data indicated that Zambian dogs were infected with multiple tick-borne pathogens such as H. canis, B. rossi, B. vogeli, A. platys, E. canis and uncharacterized Ehrlichia sp. Since some of these parasites are zoonotic, concerted efforts are needed to raise awareness of, and control, these tick-borne pathogens. |
| Rights: | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/73892 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 中尾 亮
|
