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Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia

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Title: Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia
Authors: Qiu, Yongjin Browse this author
Kaneko, Chiho Browse this author
Kajihara, Masahiro Browse this author →KAKEN DB
Ngonda, Saasa Browse this author
Simulundu, Edgar Browse this author
Muleya, Walter Browse this author
Thu, May June Browse this author
Hang'ombe, Mudenda Bernard Browse this author
Katakura, Ken Browse this author →KAKEN DB
Takada, Ayato Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Simuunza, Martin Browse this author
Nakao, Ryo Browse this author →KAKEN DB
Keywords: Anaplasmataceae
Multiple PCR
Issue Date: May-2018
Publisher: Elsevier
Journal Title: Ticks and Tick-Borne Diseases
Volume: 9
Issue: 4
Start Page: 988
End Page: 995
Publisher DOI: 10.1016/j.ttbdis.2018.03.025
Abstract: Tick-borne diseases (TBDs), including emerging and re-emerging infectious diseases, are important threats to human and animal health worldwide. Indeed, the number of reported human and animal infectious cases of novel TBD agents has increased in recent decades. However, TBDs tend to be neglected, especially in resourcelimited countries that often have limited diagnostic capacity. The aim of this molecular survey was to detect and characterise tick-borne pathogens (Babesia, Theileria, and Hepatozoon parasites and Anaplasmataceae bacteria) in domestic dogs in Zambia. In total, 247 canine peripheral blood samples were collected in Lusaka, Mazabuka, Monze, and Shangombo. Conventional PCR to detect the selected pathogens was performed using DNA extracted from canine blood. One hundred eleven samples were positive for protozoa and 5 were positive for Anaplasmataceae. Sequencing of thirty-five randomly selected protozoa-positive samples revealed the presence of Babesia rossi, Babesia vogeli, and Hepatozoon canis 18S rDNA. Based on these sequences, a multiplex PCR system was developed to yield PCR products with different amplicons, the size of which depended on the parasite species; thus, each species could be identified without the need for sequence analysis. Approximately 40% of dogs were positive for H. canis. In particular, the positive rate (75.2%) of H. canis infection was significantly higher in Shangombo than in other sampling sites. Multiplex PCR assay detected B. rossi and B. vogeli infections in five and seven dogs, respectively, indicating that this approach is useful for detecting parasites with low prevalence. Sequencing analysis of gltA and groEL genes of Anaplasmataceae revealed that two and one dogs in Lusaka were infected with Anaplasma platys and Ehrlichia canis, respectively. The data indicated that Zambian dogs were infected with multiple tick-borne pathogens such as H. canis, B. rossi, B. vogeli, A. platys, E. canis and uncharacterized Ehrlichia sp. Since some of these parasites are zoonotic, concerted efforts are needed to raise awareness of, and control, these tick-borne pathogens.
Rights: © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中尾 亮

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