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Molecular-Genetic Study on a Novel Photoperiod-Insensitive Gene in Soybean [an abstract of entire text]

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Title: Molecular-Genetic Study on a Novel Photoperiod-Insensitive Gene in Soybean [an abstract of entire text]
Other Titles: ダイズにおける新規の非感光性遺伝子に関する分子遺伝学的研究 [全文の要約]
Authors: 朱, 江慧1 Browse this author
Authors(alt): Zhu, Jianghui1
Issue Date: 25-Mar-2019
Abstract: Soybean (Glycine max (L.) Merr.) is a typical short-day plant. Eleven major genes for flowering have been reported so far. Among them, four maturity genes, E1 to E4, are the main contributors to soybean adaptation to a wide range of latitudes The floral repressor E1 is a possible transcription factor that represses the expression of major soybean FT orthologues FT2a and FT5a. E1 expression is up-regulated under long day (LD) conditions under the control of the phytochrome A (phyA) proteins E3 and E4. E1 has two homologues, E1-Like-a (E1La) and E1Lb. Down-regulation of the E1L genes by virus-induced gene silencing (VIGS) in a cultivar deficient in the E1 gene leads to early flowering and abolishes the night-break response, suggesting that the two E1L genes are also involved in the photoperiod responses of soybean. Photoperiod-insensitivity in soybean is conditioned by combinations of various alleles at E1, E3, and E4. On the basis of the functions of alleles at the three loci, Xu et al. (2013) classified ILD-insensitive cultivars into three genotypic groups: (group 1) the dysfunction of both E3 and E4; (group 2) the dysfunction of E1 in combination with that of either E3 or E4; and (group 3) a combination of e1-as (hypomorphic allele), e3, and E4. Because E4 inhibits flowering under ILD conditions, the group 3 cultivars are predicted to have novel genes that abolish or reduce ILD-sensitivity. One such gene is an early-flowering allele (FT5a-ef) at qDTF-J, a QTL for days to flowering in linkage group J, which encodes FT5a; early flowering is caused by its increased transcriptional activity or mRNA stability associated with an insertion in the promoter and/or deletions in the 3′-untranslated region (UTR). In the thesis, I studied a molecular-genetic mechanism of photoperiod-insensitivity in group 3 soybean cultivars introduced from Far-Eastern Russia. Firstly, I identified a novel loss-of-function allele at the E1Lb locus as a most likely causal factor for the photoperiod-insensitivity, by the genetic analysis including the association test, fine-mapping and sequence analysis. Secondly, I characterized the function and roles of the loss-of-function allele on flowering under LD conditions, by using near-isogenic lines. I also determined the interaction between the E1 family proteins, E1, E1La and E1Lb, by yeast two-hybrid assay. At last, I surveyed the molecular diversity for the E1La and E1Lb genes.
Description: この博士論文全文の閲覧方法については、以下のサイトをご参照ください。
Description URI: https://www.lib.hokudai.ac.jp/dissertations/copy-guides/
Conffering University: 北海道大学
Degree Report Number: 甲第13593号
Degree Level: 博士
Degree Discipline: 農学
Examination Committee Members: (主査) 教授 阿部 純, 教授 貴島 祐治, 准教授 金澤 章, 講師 山田 哲也
Degree Affiliation: 農学院(生物資源科学専攻)
Type: theses (doctoral - abstract of entire text)
URI: http://hdl.handle.net/2115/74382
Appears in Collections:課程博士 (Doctorate by way of Advanced Course) > 農学院(Graduate School of Agriculture)
学位論文 (Theses) > 博士 (農学)

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