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Factors influencing the induction of adventitious bud and callus in the brown alga Sargassum horneri (Turner) C. Agardh

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Title: Factors influencing the induction of adventitious bud and callus in the brown alga Sargassum horneri (Turner) C. Agardh
Authors: Uji, Toshiki Browse this author →KAKEN DB
Nanaumi, Daisuke Browse this author
Kawagoe, Chikara Browse this author
Saga, Naotsune Browse this author →KAKEN DB
Miyashita, Kazuo Browse this author →KAKEN DB
Keywords: Brown alga
Clonal propagation
Explant culture
Seed material
Plant growth regulators
Uniconazole
Issue Date: Aug-2016
Publisher: Springer
Journal Title: Journal of Applied Phycology
Volume: 28
Issue: 4
Start Page: 2435
End Page: 2443
Publisher DOI: 10.1007/s10811-015-0745-8
Abstract: A high frequency of callus induction and propagation from leaf explants of the brown alga Sargassum horneri was achieved within 2 months of culture when grown in medium supplemented with 5 μM uniconazole, which is a triazole-type inhibitor of cytochrome P450 enzymes. Adventitious buds were efficiently formed from the pigmented callus after transfer to medium without uniconazole, indicating that treatment with uniconazole was more beneficial for regeneration of the alga. Favorable culture conditions for induction of adventitious buds and calli included temperatures of 15 to 25 °C and light levels of 20 to 200 μmol photons m−2 s−1. Blue light promoted the production of adventitious buds and calli. The frequency of formation of adventitious buds and calli in explants from thalli with one leaf was more than 90 %, while it was 10 % only when explants were sourced from thalli with 9 to 11 leaves. These findings will be useful for clonal propagation and storage of seed materials for mariculture of Sargassum species.
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/75482
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宇治 利樹

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