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Factors influencing the induction of adventitious bud and callus in the brown alga Sargassum horneri (Turner) C. Agardh
Title: | Factors influencing the induction of adventitious bud and callus in the brown alga Sargassum horneri (Turner) C. Agardh |
Authors: | Uji, Toshiki Browse this author →KAKEN DB | Nanaumi, Daisuke Browse this author | Kawagoe, Chikara Browse this author | Saga, Naotsune Browse this author →KAKEN DB | Miyashita, Kazuo Browse this author →KAKEN DB |
Keywords: | Brown alga | Clonal propagation | Explant culture | Seed material | Plant growth regulators | Uniconazole |
Issue Date: | Aug-2016 |
Publisher: | Springer |
Journal Title: | Journal of Applied Phycology |
Volume: | 28 |
Issue: | 4 |
Start Page: | 2435 |
End Page: | 2443 |
Publisher DOI: | 10.1007/s10811-015-0745-8 |
Abstract: | A high frequency of callus induction and propagation from leaf explants of the brown alga Sargassum horneri was achieved within 2 months of culture when grown in medium supplemented with 5 μM uniconazole, which is a triazole-type inhibitor of cytochrome P450 enzymes. Adventitious buds were efficiently formed from the pigmented callus after transfer to medium without uniconazole, indicating that treatment with uniconazole was more beneficial for regeneration of the alga. Favorable culture conditions for induction of adventitious buds and calli included temperatures of 15 to 25 °C and light levels of 20 to 200 μmol photons m−2 s−1. Blue light promoted the production of adventitious buds and calli. The frequency of formation of adventitious buds and calli in explants from thalli with one leaf was more than 90 %, while it was 10 % only when explants were sourced from thalli with 9 to 11 leaves. These findings will be useful for clonal propagation and storage of seed materials for mariculture of Sargassum species. |
Rights: | The final publication is available at link.springer.com |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/75482 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宇治 利樹
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