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Involvement of l-type amino acid transporter 1 in the transport of gabapentin into human placental choriocarcinoma cells.

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Title: Involvement of l-type amino acid transporter 1 in the transport of gabapentin into human placental choriocarcinoma cells.
Authors: Furugen, Ayako Browse this author →KAKEN DB
Ishiguro, Yuri Browse this author
Kobayashi, Masaki Browse this author →KAKEN DB
Narumi, Katsuya Browse this author →KAKEN DB
Nishimura, Ayako Browse this author
Hirano, Takeshi Browse this author
Iseki, Ken Browse this author →KAKEN DB
Keywords: BeWo cells
Gabapentin (GBP)
JEG-3 cells
Organic cation/carnitine transporter (OCTN)
Placenta
l-type amino acid transporter (LAT)
Issue Date: Jan-2017
Journal Title: Reproductive toxicology (Elmsford, N.Y.)
Volume: 67
Start Page: 48
End Page: 55
Publisher DOI: 10.1016/j.reprotox.2016.11.002
PMID: 27818298
Abstract: Gabapentin (GBP) is a widely used antiepileptic drug, with potential for use in the treatment of epilepsy in pregnant women. Although studies have examined GBP transport mechanisms across the blood-brain barrier, kidney, and intestine, the mechanism in the placenta has not been fully elucidated. We previously reported that GBP accumulates at high concentrations in human placental choriocarcinoma BeWo cells. The purpose of this study was to examine the transport mechanism of GBP in placental choriocarcinoma cells (BeWo and JEG-3), and to identify the carrier involved. High concentrations of intracellular GBP accumulations were also found in JEG-3 cells. A kinetic analysis showed that a single carrier system was involved in the uptake of GBP. Furthermore, substrates for l-type amino acid transporter (LAT) and siRNAs targeted to LAT1 significantly decreased GBP uptake. Our observations from this study suggest that LAT1 is the main contributor to GBP transport in placental choriocarcinoma cells.
Rights: © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/75610
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 古堅 彩子

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