| Title: | Specificity of Morbillivirus Hemagglutinins to Recognize SLAM of Different Species |
| Authors: | Fukuhara, Hideo Browse this author |
| Ito, Yuri Browse this author |
| Sako, Miyuki Browse this author |
| Kajikawa, Mizuho Browse this author |
| Yoshida, Koki Browse this author |
| Seki, Fumio Browse this author |
| Mwaba, Mwila Hilton Browse this author |
| Hashiguchi, Takao Browse this author |
| Higashibata, Masa-aki Browse this author |
| Ose, Toyoyuki Browse this author |
| Kuroki, Kimiko Browse this author →KAKEN DB |
| Takeda, Makoto Browse this author |
| Maenaka, Katsumi Browse this author →KAKEN DB |
| Keywords: | morbillivirus |
| hemagglutinin |
| SLAM |
| canine distemper virus |
| measles virus |
| surface plasmon resonance |
| structure |
| Issue Date: | Aug-2019 |
| Publisher: | MDPI |
| Journal Title: | Viruses-Basel |
| Volume: | 11 |
| Issue: | 8 |
| Start Page: | 761 |
| Publisher DOI: | 10.3390/v11080761 |
| PMID: | 31430904 |
| Abstract: | Measles virus (MV) and canine distemper virus (CDV) are highly contagious and deadly, forming part of the morbillivirus genus. The receptor recognition by morbillivirus hemagglutinin (H) is important for determining tissue tropism and host range. Recent reports largely urge caution as regards to the potential expansion of host specificities of morbilliviruses. Nonetheless, the receptor-binding potential in different species of morbillivirus H proteins is largely unknown. Herein, we show that the CDV-H protein binds to the dog signaling lymphocyte activation molecule (SLAM), but not to the human, tamarin, or mouse SLAM. In contrast, MV-H can bind to human, tamarin and dog SLAM, but not to that of mice. Notably, MV binding to dog SLAM showed a lower affinity and faster kinetics than that of human SLAM, and MV exhibits a similar entry activity in dog SLAM- and human SLAM-expressing Vero cells. The mutagenesis study using a fusion assay, based on the MV-H-SLAM complex structure, revealed differences in tolerance for the receptor specificity between MV-H and CDV-H. These results provide insights into H-SLAM specificity related to potential host expansion. |
| Rights: | © 2019 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). |
| http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75627 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
