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Aniline derivative/DHB/alkali metal matrices for reflectron mode MALDI-TOF and TOF/TOF MS analysis of unmodified sialylated oligosaccharides and glycopeptides

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Title: Aniline derivative/DHB/alkali metal matrices for reflectron mode MALDI-TOF and TOF/TOF MS analysis of unmodified sialylated oligosaccharides and glycopeptides
Authors: Hinou, Hiroshi Browse this author →KAKEN DB
Keywords: Sialic acid
Post source decay
Alkali metal
Aniline derivative
MALDI
Pseudo MS3
Issue Date: Sep-2019
Publisher: Elsevier
Journal Title: International journal of mass spectrometry
Volume: 443
Start Page: 109
End Page: 115
Publisher DOI: 10.1016/j.ijms.2019.06.006
Abstract: Sialylglycoconjugates play biologically important roles in nature. The labile nature of the sialic acid residue in matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) analysis demands additional modification processes of the carboxylate group to avoid in-source or post-source decay. This paper reports a versatile and high-sensitivity matrix system, aniline derivative/2,5-dihydroxybenzoic acid/alkali metal salt, for MALDI-MS analysis of sialyloligosaccharides and sialylglycoconjugates without any modifications. A simple adduct ion signal was obtained from the femtomole range of the analytes tested with the new matrix system by MALDI-MS in reflectron mode. Besides, the laser power-dependent in-source decay and post-source decay pattern of the sialylated analytes with this matrix system allows a pseudo MS3 strategy for in-depth sequencing studies of the analytes. (C) 2019 The Author. Published by Elsevier B.V.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/75764
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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