|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Aniline derivative/DHB/alkali metal matrices for reflectron mode MALDI-TOF and TOF/TOF MS analysis of unmodified sialylated oligosaccharides and glycopeptides
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Aniline derivative/DHB/alkali metal matrices for reflectron mode MALDI-TOF and TOF/TOF MS analysis of unmodified sialylated oligosaccharides and glycopeptides |
| Authors: | Hinou, Hiroshi Browse this author →KAKEN DB |
| Keywords: | Sialic acid | | Post source decay | | Alkali metal | | Aniline derivative | | MALDI | | Pseudo MS3 |
| Issue Date: | Sep-2019 |
| Publisher: | Elsevier |
| Journal Title: | International journal of mass spectrometry |
| Volume: | 443 |
| Start Page: | 109 |
| End Page: | 115 |
| Publisher DOI: | 10.1016/j.ijms.2019.06.006 |
| Abstract: | Sialylglycoconjugates play biologically important roles in nature. The labile nature of the sialic acid residue in matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) analysis demands additional modification processes of the carboxylate group to avoid in-source or post-source decay. This paper reports a versatile and high-sensitivity matrix system, aniline derivative/2,5-dihydroxybenzoic acid/alkali metal salt, for MALDI-MS analysis of sialyloligosaccharides and sialylglycoconjugates without any modifications. A simple adduct ion signal was obtained from the femtomole range of the analytes tested with the new matrix system by MALDI-MS in reflectron mode. Besides, the laser power-dependent in-source decay and post-source decay pattern of the sialylated analytes with this matrix system allows a pseudo MS3 strategy for in-depth sequencing studies of the analytes. (C) 2019 The Author. Published by Elsevier B.V. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75764 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
