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An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat model

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Title: An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat model
Authors: El-naseery, Nesma Ibraheim Browse this author
Elewa, Yaser Hosny Ali Browse this author →ORCID
Ichii, Osamu Browse this author →KAKEN DB
Kon, Yasuhiro Browse this author →KAKEN DB
Keywords: Parotid gland
Ovariectomized
Mesenchymal stromal cells
Immunohistochemistry
Issue Date: Nov-2018
Publisher: Elsevier
Journal Title: Annals of anatomy - Anatomischer anzeiger
Volume: 220
Start Page: 9
End Page: 20
Publisher DOI: 10.1016/j.aanat.2018.06.006
Abstract: The current study was conducted on a menopause rat model induced by ovariectomy to assess the histological and immunohistochemical alterations in the parotid glands and to verify the efficiency of human umbilical cord derived-mesenchymal stromal cell (hUCB-MSCs) in treating this condition. Eighteen adult female rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX) and OVX injected with hUCB-MSCs (OVX + hUCB-MSCs). At 3 months post-ovariectomy, the salivary flow rate and size of the parotid glands were measured. The parotid glands were histologically investigated via H&E stained sections. Furthermore, immunohistochemical analysis for human CD105, human CD34, proliferating cell nuclear antigen (PCNA), single strand DNA (ssDNA), caspase 3, aquaporin (AQP)1, alpha-smooth muscle actin (alpha-SMA) and mouse CD34 were performed. The OVX group showed interstitial hemorrhage, dispersed acini and intracytoplasmic vacuoles in the acinar cells. Furthermore, immunohistochemical staining revealed a significant decrement in the number of ssDNA positive apoptotic cells, but a significant increment of PCNA positive proliferating cells, AQP1 positive blood capillaries, alpha-SMA positive myoepithelial cells and endogenous CD34 positive hematopoietic progenitor cells in the OVX + hUCBMSCs group as compared with the OVX group. These findings suggest a potential regenerative therapy of MSCs to injured parotid gland structures. However, further investigations are required to illustrate the mechanism of hUCB-MSCs mediated parotid gland regeneration. (C) 2018 Elsevier GmbH. All rights reserved.
Rights: © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/76001
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: ELEWA YASER HOSNY ALI

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