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An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat model
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Title: | An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat model |
Authors: | El-naseery, Nesma Ibraheim Browse this author | Elewa, Yaser Hosny Ali Browse this author →ORCID | Ichii, Osamu Browse this author →KAKEN DB | Kon, Yasuhiro Browse this author →KAKEN DB |
Keywords: | Parotid gland | Ovariectomized | Mesenchymal stromal cells | Immunohistochemistry |
Issue Date: | Nov-2018 |
Publisher: | Elsevier |
Journal Title: | Annals of anatomy - Anatomischer anzeiger |
Volume: | 220 |
Start Page: | 9 |
End Page: | 20 |
Publisher DOI: | 10.1016/j.aanat.2018.06.006 |
Abstract: | The current study was conducted on a menopause rat model induced by ovariectomy to assess the histological and immunohistochemical alterations in the parotid glands and to verify the efficiency of human umbilical cord derived-mesenchymal stromal cell (hUCB-MSCs) in treating this condition. Eighteen adult female rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX) and OVX injected with hUCB-MSCs (OVX + hUCB-MSCs). At 3 months post-ovariectomy, the salivary flow rate and size of the parotid glands were measured. The parotid glands were histologically investigated via H&E stained sections. Furthermore, immunohistochemical analysis for human CD105, human CD34, proliferating cell nuclear antigen (PCNA), single strand DNA (ssDNA), caspase 3, aquaporin (AQP)1, alpha-smooth muscle actin (alpha-SMA) and mouse CD34 were performed. The OVX group showed interstitial hemorrhage, dispersed acini and intracytoplasmic vacuoles in the acinar cells. Furthermore, immunohistochemical staining revealed a significant decrement in the number of ssDNA positive apoptotic cells, but a significant increment of PCNA positive proliferating cells, AQP1 positive blood capillaries, alpha-SMA positive myoepithelial cells and endogenous CD34 positive hematopoietic progenitor cells in the OVX + hUCBMSCs group as compared with the OVX group. These findings suggest a potential regenerative therapy of MSCs to injured parotid gland structures. However, further investigations are required to illustrate the mechanism of hUCB-MSCs mediated parotid gland regeneration. (C) 2018 Elsevier GmbH. All rights reserved. |
Rights: | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/76001 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: ELEWA YASER HOSNY ALI
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