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An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A
| Title: | An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A |
| Authors: | Mazaafrianto, Donny Nugraha Browse this author | | Ishida, Akihiko Browse this author →KAKEN DB | | Maeki, Masatoshi Browse this author | | Tani, Hirofumi Browse this author →KAKEN DB | | Tokeshi, Manabu Browse this author →KAKEN DB |
| Keywords: | Dithiol | | microfabricated device | | electrochemical detection | | aptamer | | methylene blue | | ochratoxin A | | signal-on |
| Issue Date: | 10-Nov-2019 |
| Publisher: | Japan Society for Analytical Chemistry |
| Journal Title: | Analytical sciences |
| Volume: | 35 |
| Issue: | 11 |
| Start Page: | 1221 |
| End Page: | 1226 |
| Publisher DOI: | 10.2116/analsci.19P240 |
| Abstract: | In this study, we developed an electrochemical sensor for ochratoxin A (OTA) by using an aptamer having a dithiol-based anchor, which exhibited higher stability on a gold electrode than a monothiol-based aptamer because of its two anchors. The sensor was also based on a signal-on scheme that produces a signal current resulting from structure-switching of the aptamer upon interaction with OTA. For simple fabrication of this sensor, the non-covalent interaction of methylene blue with the aptamer was also employed as an electrochemical indicator. In this study, the performance of the sensor, including the dissociation constant of the aptamer-OTA complex, was characterized. The proposed sensor exhibited high reproducibility and enough sensitivity to detect the minimum amount of OTA required for the analysis of real food samples with a limit of detection of 113 pM. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/76500 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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