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An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A

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Title: An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A
Authors: Mazaafrianto, Donny Nugraha Browse this author
Ishida, Akihiko Browse this author →KAKEN DB
Maeki, Masatoshi Browse this author
Tani, Hirofumi Browse this author →KAKEN DB
Tokeshi, Manabu Browse this author →KAKEN DB
Keywords: Dithiol
microfabricated device
electrochemical detection
aptamer
methylene blue
ochratoxin A
signal-on
Issue Date: 10-Nov-2019
Publisher: Japan Society for Analytical Chemistry
Journal Title: Analytical sciences
Volume: 35
Issue: 11
Start Page: 1221
End Page: 1226
Publisher DOI: 10.2116/analsci.19P240
Abstract: In this study, we developed an electrochemical sensor for ochratoxin A (OTA) by using an aptamer having a dithiol-based anchor, which exhibited higher stability on a gold electrode than a monothiol-based aptamer because of its two anchors. The sensor was also based on a signal-on scheme that produces a signal current resulting from structure-switching of the aptamer upon interaction with OTA. For simple fabrication of this sensor, the non-covalent interaction of methylene blue with the aptamer was also employed as an electrochemical indicator. In this study, the performance of the sensor, including the dissociation constant of the aptamer-OTA complex, was characterized. The proposed sensor exhibited high reproducibility and enough sensitivity to detect the minimum amount of OTA required for the analysis of real food samples with a limit of detection of 113 pM.
Type: article
URI: http://hdl.handle.net/2115/76500
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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