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Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts

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Title: Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts
Authors: Yamamoto, Kei Browse this author
Otomo, Kohei Browse this author →KAKEN DB
Nemoto, Tomomi Browse this author →KAKEN DB
Ishihara, Seiichiro Browse this author →KAKEN DB
Haga, Hisashi Browse this author →KAKEN DB
Nagasaki, Akira Browse this author →KAKEN DB
Murakami, Yota Browse this author →KAKEN DB
Takahashi, Masayuki Browse this author →KAKEN DB
Keywords: Nonmuscle myosin IIA
Nonmuscle myosin IlB
Contractile ring
Three-dimensional imaging
Cortical stiffness
Issue Date: 1-Mar-2019
Publisher: Elsevier
Journal Title: Experimental cell research
Volume: 376
Issue: 1
Start Page: 67
End Page: 76
Publisher DOI: 10.1016/j.yexcr.2019.01.020
Abstract: Nonmuscle myosin II (NMII) plays an important role in cytokinesis by constricting a contractile ring. However, it is poorly understood how NMII isoforms contribute to cytokinesis in mammalian cells. Here, we investigated the roles of the two major NMII isoforms, NMIIA and NMIIB, in cytokinesis using a WI-38 VA13 cell line (human immortalized fibroblast). In this cell line, NMIIB tended to localize to the contractile ring more than NMIIA. The expression level of NMIIA affected the localization of NMIIB. Most NMIIB accumulated at the cleavage furrow in NMIIA-knockout (KO) cells, and most NMIIA was displaced from this location in exogenous NMIIB-expressing cells, indicating that NMIIB preferentially localizes to the contractile ring. Specific KO of each isoform elicited opposite effects. The rate of furrow ingression was decreased and increased in NMIIA-KO and NMIIB-KO cells, respectively. Meanwhile, the length of NMII-filament stacks in the contractile ring was increased and decreased in NMIIA-KO and NMIIB-KO cells, respectively. Moreover, NMIIA helped to maintain cortical stiffness during cytokinesis. These findings suggest that appropriate ratio of NMIIA and NMIIB in the contractile ring is important for proper cytokinesis in specific cell types. In addition, two-photon excitation spinning-disk confocal microscopy enabled us to image constriction of the contractile ring in live cells in a three-dimensional manner.
Rights: ©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 正行

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