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Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts
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| Title: | Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts |
| Authors: | Yamamoto, Kei Browse this author | | Otomo, Kohei Browse this author →KAKEN DB | | Nemoto, Tomomi Browse this author →KAKEN DB | | Ishihara, Seiichiro Browse this author →KAKEN DB | | Haga, Hisashi Browse this author →KAKEN DB | | Nagasaki, Akira Browse this author →KAKEN DB | | Murakami, Yota Browse this author →KAKEN DB | | Takahashi, Masayuki Browse this author →KAKEN DB |
| Keywords: | Nonmuscle myosin IIA | | Nonmuscle myosin IlB | | Contractile ring | | Cytokinesis | | Three-dimensional imaging | | Cortical stiffness |
| Issue Date: | 1-Mar-2019 |
| Publisher: | Elsevier |
| Journal Title: | Experimental cell research |
| Volume: | 376 |
| Issue: | 1 |
| Start Page: | 67 |
| End Page: | 76 |
| Publisher DOI: | 10.1016/j.yexcr.2019.01.020 |
| Abstract: | Nonmuscle myosin II (NMII) plays an important role in cytokinesis by constricting a contractile ring. However, it is poorly understood how NMII isoforms contribute to cytokinesis in mammalian cells. Here, we investigated the roles of the two major NMII isoforms, NMIIA and NMIIB, in cytokinesis using a WI-38 VA13 cell line (human immortalized fibroblast). In this cell line, NMIIB tended to localize to the contractile ring more than NMIIA. The expression level of NMIIA affected the localization of NMIIB. Most NMIIB accumulated at the cleavage furrow in NMIIA-knockout (KO) cells, and most NMIIA was displaced from this location in exogenous NMIIB-expressing cells, indicating that NMIIB preferentially localizes to the contractile ring. Specific KO of each isoform elicited opposite effects. The rate of furrow ingression was decreased and increased in NMIIA-KO and NMIIB-KO cells, respectively. Meanwhile, the length of NMII-filament stacks in the contractile ring was increased and decreased in NMIIA-KO and NMIIB-KO cells, respectively. Moreover, NMIIA helped to maintain cortical stiffness during cytokinesis. These findings suggest that appropriate ratio of NMIIA and NMIIB in the contractile ring is important for proper cytokinesis in specific cell types. In addition, two-photon excitation spinning-disk confocal microscopy enabled us to image constriction of the contractile ring in live cells in a three-dimensional manner. |
| Rights: | ©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/76831 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高橋 正行
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