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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
| Title: | Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species |
| Authors: | Kawai, Yusuke K. Browse this author | | Shinya, So Browse this author | | Ikenaka, Yoshinori Browse this author | | Saengtienchai, Aksorn Browse this author | | Kondo, Takamitsu Browse this author | | Darvvish, Wageh Sobhy Browse this author | | Nakayama, Shota M. M. Browse this author | | Mizukawa, Hazuki Browse this author →KAKEN DB | | Ishizuka, Mayumi Browse this author →KAKEN DB |
| Keywords: | UGTs | | Xenobiotic | | Bird | | Wildlife | | Toxicology |
| Issue Date: | Mar-2019 |
| Publisher: | Elsevier |
| Journal Title: | Comparative biochemistry and physiology Part C: Toxicology & pharmacology |
| Volume: | 217 |
| Start Page: | 5 |
| End Page: | 14 |
| Publisher DOI: | 10.1016/j.cbpc.2018.11.001 |
| Abstract: | Birds are exposed to many xenobiotics during their lifetime. For accurate prediction of xenobiotic-induced toxic effects on avian species, it is necessary to understand metabolic capacities in a comprehensive range of bird species. However, there is a lack of information about avian xenobiotic metabolizing enzymes (XMEs), particularly in wild birds. Uridine diphosphate glucuronosyltransferase (UGT) is an XME that plays an important role in phase II metabolism in the livers of mammals and birds. This study was performed to determine the characteristics of UGT1E isoform in avian species, those are related to mammals UGT 1A. To understand the characteristics of avian UGT1E isoforms, in vitro metabolic activity and genetic characteristics were investigated. Furthermore, mRNA expression levels of all chicken UGT1E isoforms were measured. On in vitro enzymatic analysis, the white-tailed eagle, great horned owl, and Humboldt penguin showed lower UGT-dependent activity than domestic birds. In synteny analysis, carnivorous birds were shown to have fewer UGT1E isoforms than herbivorous and omnivorous birds, which may explain why they have lower in vitro UGT activity. These observations suggested that raptors and seabirds, in which UGT activity is low, may be at high risk if exposed to elevated levels of xenobiotics in the environment. Phylogenetic analysis suggested that avian UGT1Es have evolved independently from mammalian UGT1As. We identified the important UGT isoforms, such as UGT1E13, and suspected their substrate specificities in avian xenobiotic metabolism by phylogenetic and quantitative realtime PCR analysis. This is the first report regarding the genetic characteristics and interspecies differences of UGT1Es in avian species. |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/76849 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石塚 真由美
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