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Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species

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Title: Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species
Authors: Kawai, Yusuke K. Browse this author
Shinya, So Browse this author
Ikenaka, Yoshinori Browse this author
Saengtienchai, Aksorn Browse this author
Kondo, Takamitsu Browse this author
Darvvish, Wageh Sobhy Browse this author
Nakayama, Shota M. M. Browse this author
Mizukawa, Hazuki Browse this author →KAKEN DB
Ishizuka, Mayumi Browse this author →KAKEN DB
Keywords: UGTs
Issue Date: Mar-2019
Publisher: Elsevier
Journal Title: Comparative biochemistry and physiology Part C: Toxicology & pharmacology
Volume: 217
Start Page: 5
End Page: 14
Publisher DOI: 10.1016/j.cbpc.2018.11.001
Abstract: Birds are exposed to many xenobiotics during their lifetime. For accurate prediction of xenobiotic-induced toxic effects on avian species, it is necessary to understand metabolic capacities in a comprehensive range of bird species. However, there is a lack of information about avian xenobiotic metabolizing enzymes (XMEs), particularly in wild birds. Uridine diphosphate glucuronosyltransferase (UGT) is an XME that plays an important role in phase II metabolism in the livers of mammals and birds. This study was performed to determine the characteristics of UGT1E isoform in avian species, those are related to mammals UGT 1A. To understand the characteristics of avian UGT1E isoforms, in vitro metabolic activity and genetic characteristics were investigated. Furthermore, mRNA expression levels of all chicken UGT1E isoforms were measured. On in vitro enzymatic analysis, the white-tailed eagle, great horned owl, and Humboldt penguin showed lower UGT-dependent activity than domestic birds. In synteny analysis, carnivorous birds were shown to have fewer UGT1E isoforms than herbivorous and omnivorous birds, which may explain why they have lower in vitro UGT activity. These observations suggested that raptors and seabirds, in which UGT activity is low, may be at high risk if exposed to elevated levels of xenobiotics in the environment. Phylogenetic analysis suggested that avian UGT1Es have evolved independently from mammalian UGT1As. We identified the important UGT isoforms, such as UGT1E13, and suspected their substrate specificities in avian xenobiotic metabolism by phylogenetic and quantitative realtime PCR analysis. This is the first report regarding the genetic characteristics and interspecies differences of UGT1Es in avian species.
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石塚 真由美

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