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Forced isoform switching of Neat1_1 to Neat1_2 leads to the loss of Neat1_1 and the hyperformation of paraspeckles but does not affect the development and growth of mice

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Title: Forced isoform switching of Neat1_1 to Neat1_2 leads to the loss of Neat1_1 and the hyperformation of paraspeckles but does not affect the development and growth of mice
Authors: Isobe, Momo Browse this author
Toya, Hikaru Browse this author
Mito, Mari Browse this author
Chiba, Tomoki Browse this author
Asahara, Hiroshi Browse this author
Hirose, Tetsuro Browse this author
Nakagawa, Shinichi Browse this author →KAKEN DB
Keywords: Neat1
paraspeckles
genome editing
isoform
mouse
Issue Date: Mar-2020
Publisher: Cold Spring Harbor Laboratory Press
Journal Title: RNA
Volume: 26
Issue: 3
Start Page: 251
End Page: 264
Publisher DOI: 10.1261/rna.072587.119
Abstract: Neat1 is a long noncoding RNA (lncRNA) that serves as an architectural component of the nuclear bodies known as para-speckles. Two isoforms of Neat1, the short isoform Neat1_1 and the long isoform Neat1_2, are generated from the same gene locus by alternative 3' processing. Neat1_1 is the most abundant and the best conserved isoform expressed in various cell types, whereas Neat1_2 is expressed in a small population of particular cell types, including the tip cells of the intestinal epithelium. To investigate the physiological significance of isoform switching, we created mutant mice that solely expressed Neat1_2 by deleting the upstream polyadenylation ( poly-A) signal (PAS) required for the production of Neat1_1. We observed the loss of Neat1_1 and strong up-regulation of Neat1_2 in various tissues and cells and the subsequent hyperformation of paraspeckles, especially in cells that normally express Neat1_2. However, the mutant mice were born at the expected Mendelian ratios and did not exhibit obvious external and histological abnormalities. These observations suggested that the hyperformation of paraspeckles does not interfere with the development and growth of these animals under normal laboratory conditions.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/77023
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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