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Elucidation of biosynthetic pathway of a plant hormone abscisic acid in phytopathogenic fungi

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Title: Elucidation of biosynthetic pathway of a plant hormone abscisic acid in phytopathogenic fungi
Authors: Takino, Junya Browse this author
Kozaki, Takuto Browse this author
Ozaki, Taro Browse this author
Liu, Chengwei Browse this author
Minami, Atsushi Browse this author
Oikawa, Hideaki Browse this author
Keywords: Abscisic acid
plant hormone
oxidative modifications
Aspergillus oryzae
phytopathogenic fungi
Issue Date: 21-May-2020
Publisher: Taylor & Francis
Journal Title: Bioscience biotechnology and biochemistry
Volume: 83
Issue: 9
Start Page: 1642
End Page: 1649
Publisher DOI: 10.1080/09168451.2019.1618700
Abstract: Abscisic acid (ABA) is one of the plant hormones that regulates physiological functions in various organisms, including plants, sponges, and humans. The biosynthetic machinery in plants is firmly established, while that in fungi is still unclear. Here, we elucidated the functions of the four biosynthetic genes, bcABA1-bcABA4, found in Botrytis cinerea by performing biotransformation experiments and in vitro enzymatic reactions with putative biosynthetic intermediates. The first-committed step is the cyclization of farnesyl diphosphate to give alpha-ionylideneethane catalyzed by a novel sesquiterpene synthase, BcABA3, which exhibits low amino acid sequence identities with sesquiterpene synthases. Subsequently, two cytochrome P450s, BcABA1 and BcABA2, mediate oxidative modifications of the cyclized product to afford 1MODIFIER LETTER PRIME,4MODIFIER LETTER PRIME-trans-dihydroxy-alpha-ionylideneacetic acid, which undergoes alcohol oxidation to furnish ABA. Our results demonstrated that production of ABA does not depend on the nucleotide sequence of bcABA genes. The present study set the stage to investigate the role of ABA in infections.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 21 May 2019, available online:
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 及川 英秋

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