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Improvement of Electrochemical Conditions for Detecting Redox Reaction of K-3[Fe(CN)(6)] toward the Application in Norovirus Aptasensor
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| Title: | Improvement of Electrochemical Conditions for Detecting Redox Reaction of K-3[Fe(CN)(6)] toward the Application in Norovirus Aptasensor |
| Authors: | Hirano, Seiya Browse this author | | Saito, Junki Browse this author | | Yukawa, Tomoki Browse this author | | Sano, Daisuke Browse this author →KAKEN DB | | Okamoto, Akihiro Browse this author | | Okabe, Satoshi Browse this author →KAKEN DB | | Kitajima, Masaaki Browse this author |
| Keywords: | Norovirus | | Aptamer | | Screen-printed Electrode | | Biosensor |
| Issue Date: | 14-Apr-2020 |
| Publisher: | The Electrochemical Society of Japan |
| Journal Title: | Electrochemistry |
| Volume: | 88 |
| Issue: | 3 |
| Start Page: | 205 |
| End Page: | 209 |
| Publisher DOI: | 10.5796/electrochemistry.20-00017 |
| Abstract: | Electrochemical biosensors have attracted significant attention as a novel tool for the sensitive detection of pathogens and contaminants, with the potential capability of rapid, on-site diagnosis. In this study, we intended to improve the sensitivity of electrochemical biosensor using Fe(CN)(6)(3)(-/)(4-) as redox marker, toward its application to norovirus aptasensors. Although many researchers have developed electrochemical aptasensors for various analytes using redox markers, the reported electrochemical conditions under which an aptasensor was examined varied across multiple studies. Here, we performed square-wave voltammetry (SWV) for electrodes modified with aptamer specific to murine norovirus and compared the reduction peak currents of Fe(CN)(6)(3-) under various conditions. Effects of working electrode materials and NaCI and [Ru(NH3)(6)]Cl-3 concentration in the electrolyte were examined. Among conditions we tested, the best sensitivity was obtained using a screen-printed gold electrode in an electrolyte containing 1M NaCl with 4 mM K-3[Fe(CN)(6)], in which the concentration of murine norovirus showed linearity with SWV peak current. This study provided useful information on the electrochemical measurement conditions regarding the development of electrochemical aptasensors. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/78391 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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