| Title: | Protein Phosphorylation Dynamics Under Carbon/Nitrogen-Nutrient Stress and Identification of a Cell Death-Related Receptor-Like Kinase in Arabidopsis |
| Authors: | Li, Xingwen Browse this author |
| Sanagi, Miho Browse this author |
| Lu, Yu Browse this author |
| Nomura, Yuko Browse this author |
| Stolze, Sara Christina Browse this author |
| Yasuda, Shigetaka Browse this author |
| Saijo, Yusuke Browse this author |
| Schulze, Waltraud X. Browse this author |
| Feil, Regina Browse this author |
| Stitt, Mark Browse this author |
| Lunn, John E. Browse this author |
| Nakagami, Hirofumi Browse this author |
| Sato, Takeo Browse this author |
| Yamaguchi, Junji Browse this author →KAKEN DB |
| Keywords: | sugar |
| nitrogen |
| phosphorylation |
| kinase |
| SnRK1 |
| receptor-like kinase |
| metabolism |
| cell death |
| Issue Date: | 3-Apr-2020 |
| Publisher: | Frontiers Media |
| Journal Title: | Frontiers in plant science |
| Volume: | 11 |
| Start Page: | 377 |
| Publisher DOI: | 10.3389/fpls.2020.00377 |
| Abstract: | Nutrient availability, in particular the availability of sugar [carbon (C)] and nitrogen (N), is important for the regulation of plant metabolism and development. In addition to independent utilization of C and N nutrients, plants sense and respond to the balance of C and N nutrients (C/N-nutrient) available to them. High C/low N-nutrient stress has been shown to arrest early post-germinative growth while promoting progression to senescence in Arabidopsis. Although several signaling components of the C/N-nutrient response have been identified, the inclusive molecular basis of plant C/N-nutrient response remains unclear. This proteome analysis evaluated phosphorylation dynamics in response to high C/low N-nutrient stress. Phosphoproteomics under conditions of C/N-nutrient stress showed a global change in the phosphorylation status of proteins, including plasma membrane H+-ATPase, carbon and nitrogen metabolic enzymes and signaling proteins such as protein kinases and transcription factors. Further analyses suggested that SNF1-related protein kinase 1 (SnRK1) is involved in primary C/N-nutrient signal mediation via the transcriptional regulation of C/N-regulatory kinases. We also identified a leucine-rich repeat receptor-like kinase with extracellular malectin-like domain, named as LMK1, which was shown to possess cell death induction activity in plant leaves. These results provide important insight into the C/N-nutrient signaling pathways connecting nutrition stress to various cellular and physiological processes in plants. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/78408 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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