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Hokkaido University Collection of Scholarly and Academic Papers >
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Peer-reviewed Journal Articles, etc >
Involvement of Peptide Epimerization in Poly-gamma-glutamic Acid Biosynthesis
| Title: | Involvement of Peptide Epimerization in Poly-gamma-glutamic Acid Biosynthesis |
| Authors: | Ogasawara, Yasushi Browse this author | | Shigematsu, Mayuko Browse this author | | Sato, Shota Browse this author | | Kato, Hinata Browse this author | | Dairi, Tohru Browse this author →KAKEN DB |
| Issue Date: | 7-Jun-2019 |
| Publisher: | American Chemical Society |
| Journal Title: | Organic letters |
| Volume: | 21 |
| Issue: | 11 |
| Start Page: | 3972 |
| End Page: | 3975 |
| Publisher DOI: | 10.1021/acs.orglett.9b01121 |
| Abstract: | Poly-gamma-glutamic acid (PGA) is a promising polymer that comprises D- and L-glutamic acid (Glu) connected via an amide bond. PGA is biosynthesized by a transmembrane enzyme complex composed of PgsB, PgsC, and PgsA. However, the detailed reaction, especially the mechanism for introducing D-Glu residues into PGA, remains elusive. We herein report isotope tracer experiments with deuterated L- and D-Glu and demonstrate that epimerization of a growing peptide is involved in PGA biosynthesis. |
| Rights: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Organic Letters, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.orglett.9b01121. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/78446 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 小笠原 泰志
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