HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >

Radial alignment of microtubules through tubulin polymerization in an evaporating droplet

This item is licensed under: Creative Commons Attribution 4.0 International

Files in This Item:

The file(s) associated with this item can be obtained from the following URL:https://doi.org/10.1371/journal.pone.0231352


Title: Radial alignment of microtubules through tubulin polymerization in an evaporating droplet
Authors: Keya, Jakia Jannat Browse this author
Kudoh, Hiroki Browse this author
Kabir, Arif Md. Rashedul Browse this author
Inoue, Daisuke Browse this author
Miyamoto, Nobuyoshi Browse this author
Tani, Tomomi Browse this author
Kakugo, Akira Browse this author →KAKEN DB
Shikinaka, Kazuhiro Browse this author
Issue Date: 10-Apr-2020
Publisher: PLOS
Journal Title: PLoS ONE
Volume: 15
Issue: 4
Start Page: e0231352
Publisher DOI: 10.1371/journal.pone.0231352
Abstract: We report the formation of spherulites from droplets of highly concentrated tubulin solution via nucleation and subsequent polymerization to microtubules (MTs) under water evaporation by heating. Radial alignment of MTs in the spherulites was confirmed by the optical properties of the spherulites observed using polarized optical microscopy and fluorescence microscopy. Temperature and concentration of tubulins were found as important parameters to control the spherulite pattern formation of MTs where evaporation plays a significant role. The alignment of MTs was regulated reversibly by temperature induced polymerization and depolymerization of tubulins. The formation of the MTs patterns was also confirmed at the molecular level from the small angle X-ray measurements. This work provides a simple method for obtaining radially aligned arrays of MTs.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/78736
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University