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A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose

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Title: A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose
Authors: Togo, Yuki Browse this author
Nakashima, Kazunori Browse this author
Mwandira, Wilson Browse this author
Kawasaki, Satoru Browse this author →KAKEN DB
Keywords: Carbohydrate-binding module
immobilization
metal affinity tag
transition metals
Issue Date: Apr-2020
Publisher: 日本分析化学会(Japan Society for Analytical Chemistry)
Journal Title: Analytical sciences
Volume: 36
Issue: 4
Start Page: 459
End Page: 464
Publisher DOI: 10.2116/analsci.19P356
Abstract: We developed a novel metal adsorbent composed of bio-based materials, cellulose and a protein. The approach involved the immobilization of a hexa-histidine tag (His(6)), which shows an affinity for an intermediate acid (metal ion) in Hard and Soft Acids and Bases (HSAB) theory, on cellulose by fusing with a carbohydrate-binding module (CBM). The results show that CBM-His(6)-bound cellulose has adsorption selectivity reflecting the original properties of His(6). Additionally, we prepared three configurations of CBM-His(6) proteins, which were subsequently immobilized on filter paper for Ni2+ ion adsorption. Of these configurations, we found that the protein containing two His(6) tags at each terminus (N- and C-) of CBM exhibited the highest metal adsorption ability. Furthermore, XPS analysis confirmed the binding of Ni2+ ions on the cellulose.
Type: article
URI: http://hdl.handle.net/2115/78992
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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