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In Vitro Enzymatic Activity Assays Implicate the Existence of the Chlorophyll Cycle in Chlorophyll b-Containing Cyanobacteria

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/79039

Title: In Vitro Enzymatic Activity Assays Implicate the Existence of the Chlorophyll Cycle in Chlorophyll b-Containing Cyanobacteria
Authors: Lim, HyunSeok Browse this author
Tanaka, Ayumi Browse this author →KAKEN DB
Tanaka, Ryouichi Browse this author →KAKEN DB
Ito, Hisashi Browse this author
Keywords: Chlorophyll
Cyanobacterium
Enzyme
Evolution
Promiscuous activity.
Issue Date: Aug-2019
Publisher: Oxford University Press
Journal Title: Plant and Cell Physiology
Volume: 60
Issue: 12
Start Page: 2672
End Page: 2683
Publisher DOI: 10.1093/pcp/pcz157
Abstract: In plants, chlorophyll (Chl) a and b are interconvertible by the action of three enzymes-chlorophyllide a oxygenase, Chl b reductase (CBR) and 7-hydroxymethyl chlorophyll a reductase (HCAR). These reactions are collectively referred to as the Chl cycle. In plants, this cyclic pathway ubiquitously exists and plays essential roles in acclimation to different light conditions at various developmental stages. By contrast, only a limited number of cyanobacteria species produce Chl b, and these include Prochlorococcus, Prochloron, Prochlorothrix and Acaryochloris. In this study, we investigated a possible existence of the Chl cycle in Chl b synthesizing cyanobacteria by testing in vitro enzymatic activities of CBR and HCAR homologs from Prochlorothrix hollandica and Acaryochloris RCC1774. All of these proteins show respective CBR and HCAR activity in vitro, indicating that both cyanobacteria possess the potential to complete the Chl cycle. It is also found that CBR and HCAR orthologs are distributed only in the Chl b-containing cyanobacteria that habitat shallow seas or freshwater, where light conditions change dynamically, whereas they are not found in Prochlorococcus species that usually habitat environments with fixed lighting. Taken together, our results implicate a possibility that the Chl cycle functions for light acclimation in Chl b-containing cyanobacteria.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in Plant and Cell Physiology following peer review. The version of record [HyunSeok Lim, Ayumi Tanaka, Ryouichi Tanaka, Hisashi Ito, In Vitro Enzymatic Activity Assays Implicate the Existence of the Chlorophyll Cycle in Chlorophyll b-Containing Cyanobacteria, Plant and Cell Physiology, Volume 60, Issue 12, December 2019, Pages 2672–2683] is available online at: https://academic.oup.com/pcp/article/60/12/2672/5544940.
Type: article (author version)
URI: http://hdl.handle.net/2115/79039
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伊藤 寿

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