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Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines

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Title: Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines
Authors: Kawamoto, Masaki Browse this author
Yamaji, Toshiyuki Browse this author
Saito, Kyoko Browse this author
Shirasago, Yoshitaka Browse this author
Satomura, Kazuhiro Browse this author
Endo, Toshinori Browse this author
Fukasawa, Masayoshi Browse this author
Hanada, Kentaro Browse this author
Osada, Naoki Browse this author
Keywords: Huh7 cell line
genome sequencing
cell lines
hepatitis C virus
cell substrate
Issue Date: 9-Oct-2020
Publisher: Frontiers Media
Journal Title: Frontiers in Genetics
Volume: 11
Start Page: 546106
Publisher DOI: 10.3389/fgene.2020.546106
Abstract: The human hepatoma-derived HuH-7 cell line and its derivatives (Huh7.5 and Huh7.5.1) have been widely used as a convenient experimental substitute for primary hepatocytes. In particular, these cell lines represent host cells suitable for propagating the hepatitis C virus (HCV) in vitro. The Huh7.5.1-8 cell line, a subline of Huh7.5.1, can propagate HCV more efficiently than its parental cells. To provide genomic information for cells' quality control, we performed whole-genome sequencing of HuH-7 and Huh7.5.1-8 and identified their characteristic genomic deletions, some of which are applicable to an in-house test for cell authentication. Among the genes related to HCV infection and replication, 53 genes were found to carry missense or loss-of-function mutations likely specific to the HuH-7 and/or Huh7.5.1-8. Eight genes, including DDX58 (RIG-I), BAX, EP300, and SPP1 (osteopontin), contained mutations observed only in Huh7.5.1-8 or mutations with higher frequency in Huh7.5.1-8. These mutations might be relevant to phenotypic differences between the two cell lines and may also serve as genetic markers to distinguish Huh7.5.1-8 cells from the ancestral HuH-7 cells.
Type: article
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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