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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Information Science and Technology / Faculty of Information Science and Technology >
Peer-reviewed Journal Articles, etc >
Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines |
| Authors: | Kawamoto, Masaki Browse this author | | Yamaji, Toshiyuki Browse this author | | Saito, Kyoko Browse this author | | Shirasago, Yoshitaka Browse this author | | Satomura, Kazuhiro Browse this author | | Endo, Toshinori Browse this author | | Fukasawa, Masayoshi Browse this author | | Hanada, Kentaro Browse this author | | Osada, Naoki Browse this author →KAKEN DB |
| Keywords: | Huh7 cell line | | genome sequencing | | cell lines | | hepatitis C virus | | cell substrate |
| Issue Date: | 9-Oct-2020 | | 9-Oct-2020 |
| Publisher: | Frontiers Media |
| Journal Title: | Frontiers in Genetics |
| Volume: | 11 |
| Start Page: | 546106 |
| Publisher DOI: | 10.3389/fgene.2020.546106 |
| Abstract: | The human hepatoma-derived HuH-7 cell line and its derivatives (Huh7.5 and Huh7.5.1) have been widely used as a convenient experimental substitute for primary hepatocytes. In particular, these cell lines represent host cells suitable for propagating the hepatitis C virus (HCV) in vitro. The Huh7.5.1-8 cell line, a subline of Huh7.5.1, can propagate HCV more efficiently than its parental cells. To provide genomic information for cells' quality control, we performed whole-genome sequencing of HuH-7 and Huh7.5.1-8 and identified their characteristic genomic deletions, some of which are applicable to an in-house test for cell authentication. Among the genes related to HCV infection and replication, 53 genes were found to carry missense or loss-of-function mutations likely specific to the HuH-7 and/or Huh7.5.1-8. Eight genes, including DDX58 (RIG-I), BAX, EP300, and SPP1 (osteopontin), contained mutations observed only in Huh7.5.1-8 or mutations with higher frequency in Huh7.5.1-8. These mutations might be relevant to phenotypic differences between the two cell lines and may also serve as genetic markers to distinguish Huh7.5.1-8 cells from the ancestral HuH-7 cells. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/79830 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 長田 直樹
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