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Subtle Control of Carbon Chain Length in Polyunsaturated Fatty Acid Synthases
| Title: | Subtle Control of Carbon Chain Length in Polyunsaturated Fatty Acid Synthases |
| Authors: | Nakat, Mai Browse this author | | Ikeuchi, Kenshin Browse this author | | Hayashi, Shohei Browse this author | | Satoh, Yasuharu Browse this author →KAKEN DB | | Ogasawara, Yasushi Browse this author | | Dairi, Tohru Browse this author →KAKEN DB |
| Keywords: | Polyunsaturated fatty acid | | biosynthesis | | enzymes | | mutagenesis |
| Issue Date: | Dec-2019 |
| Publisher: | American Chemical Society |
| Journal Title: | ACS chemical biology |
| Volume: | 14 |
| Issue: | 12 |
| Start Page: | 2553 |
| End Page: | 2556 |
| Publisher DOI: | 10.1021/acschembio.9b00803 |
| Abstract: | Some marine bacteria synthesize docosahexaenoic acid (DHA; C-22) and eicosapentaenoic acid (EPA; C-20) by enzyme complexes composed of four subunits (A-D). We recently revealed that beta-ketoacyl synthase (KSC)/chain length factor (CLF)-like domains in the "C" subunit of DHA synthase catalyzed the last elongation step (C-20 to C-22) even though their amino acid sequences are very similar to those of EPA synthase. To investigate the amino acid residues controlling the product chain length, conserved residues in the KSC/CLF-like domains in DHA synthase were replaced with corresponding EPA synthase residues. Among 12 mutants, two CLF-like domain-mutated genes completely lost DHA productivity and produced trace amounts of EPA when coexpressed with dha-ABD in Escherichia coli, whereas when coexpressed with epa-ABD, they produced the same amounts of EPA as epa-ABCD. These results suggest that the product profiles were subtly controlled by several amino acid residues. |
| Rights: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS chemical biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acschembio.9b00803. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/79863 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 大利 徹
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