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Subtle Control of Carbon Chain Length in Polyunsaturated Fatty Acid Synthases

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Title: Subtle Control of Carbon Chain Length in Polyunsaturated Fatty Acid Synthases
Authors: Nakat, Mai Browse this author
Ikeuchi, Kenshin Browse this author
Hayashi, Shohei Browse this author
Satoh, Yasuharu Browse this author →KAKEN DB
Ogasawara, Yasushi Browse this author →KAKEN DB
Dairi, Tohru Browse this author →KAKEN DB
Keywords: Polyunsaturated fatty acid
Issue Date: Dec-2019
Publisher: American Chemical Society
Journal Title: ACS chemical biology
Volume: 14
Issue: 12
Start Page: 2553
End Page: 2556
Publisher DOI: 10.1021/acschembio.9b00803
Abstract: Some marine bacteria synthesize docosahexaenoic acid (DHA; C-22) and eicosapentaenoic acid (EPA; C-20) by enzyme complexes composed of four subunits (A-D). We recently revealed that beta-ketoacyl synthase (KSC)/chain length factor (CLF)-like domains in the "C" subunit of DHA synthase catalyzed the last elongation step (C-20 to C-22) even though their amino acid sequences are very similar to those of EPA synthase. To investigate the amino acid residues controlling the product chain length, conserved residues in the KSC/CLF-like domains in DHA synthase were replaced with corresponding EPA synthase residues. Among 12 mutants, two CLF-like domain-mutated genes completely lost DHA productivity and produced trace amounts of EPA when coexpressed with dha-ABD in Escherichia coli, whereas when coexpressed with epa-ABD, they produced the same amounts of EPA as epa-ABCD. These results suggest that the product profiles were subtly controlled by several amino acid residues.
Rights: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS chemical biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大利 徹

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