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The effect of zebularine on the heat-activated retrotransposon ONSEN in Arabidopsis thaliana and Vigna angularis

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Title: The effect of zebularine on the heat-activated retrotransposon ONSEN in Arabidopsis thaliana and Vigna angularis
Authors: Boonjing, Patwira Browse this author
Masuta, Yukari Browse this author
Nozawa, Kosuke Browse this author
Kato, Atsushi Browse this author
Ito, Hidetaka Browse this author →KAKEN DB
Keywords: adzuki bean
Arabidopsis
heat stress
ONSEN
zebularine
Issue Date: Aug-2020
Publisher: 日本遺伝学会(The Genetics Society of Japan)
Journal Title: Genes & Genetic Systems
Volume: 95
Issue: 4
Start Page: 165
End Page: 172
Publisher DOI: 10.1266/ggs.19-00046
Abstract: The Ty1/copia-like retrotransposon ONSEN is conserved among Brassica species, as well as in beans, including adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi), which is one of the economically important crops in Japan. ONSEN has acquired a heat-responsive element that is recognized by plant heat stress defense factors, resulting in its transcription and the production of full-length extrachromosomal DNA under conditions with elevated temperatures. DNA methylation plays an important role in regulating the activation of this transposon in plants. Therefore, chemical inhibition of DNA methyltransferases has been utilized to study the effect of DNA methylation on transposon activation. To understand the effect of DNA methylation on ONSEN activation, Arabidopsis thaliana and adzuki bean seedlings were treated with zebularine, which is known to be an effective chemical demethylation agent. The results showed that ONSEN transcription levels were upregulated in zebularine-treated plants. Extrachromosomal DNA of ONSEN also accumulated in the treated plants.
Type: article
URI: http://hdl.handle.net/2115/79977
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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