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Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli
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| Title: | Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli |
| Authors: | Arai, Shuzo Browse this author | | Sakakibara, Sayaka Browse this author | | Mareschal, Robin Browse this author | | Ooi, Toshihiko Browse this author →KAKEN DB | | Zinn, Manfred Browse this author | | Matsumoto, Ken'ichiro Browse this author →KAKEN DB |
| Keywords: | sequence regulation | | block copolymer | | engineered PHA synthase | | polyglycolic acid | | chimeric enzyme |
| Issue Date: | 8-Dec-2020 |
| Publisher: | Frontiers Media |
| Journal Title: | Frontiers in bioengineering and biotechnology |
| Volume: | 8 |
| Start Page: | 612991 |
| Publisher DOI: | 10.3389/fbioe.2020.612991 |
| Abstract: | Glycolate (GL)-containing polyhydroxyalkanoate (PHA) was synthesized in Escherichia coli expressing the engineered chimeric PHA synthase PhaC(AR) and coenzyme A transferase. The cells produced poly[GL-co-3-hydroxybutyrate (3HB)] with the supplementation of GL and 3HB, thus demonstrating that PhaC(AR) is the first known class I PHA synthase that is capable of incorporating GL units. The triad sequence analysis using H-1 nuclear magnetic resonance indicated that the obtained polymer was composed of two distinct regions, a P(GL-ran-3HB) random segment and P(3HB) homopolymer segment. The random segment was estimated to contain a 71 mol% GL molar ratio, which was much greater than the value (15 mol%) previously achieved by using PhaC1(P)(s)STQK. Differential scanning calorimetry analysis of the polymer films supported the presence of random copolymer and homopolymer phases. The solvent fractionation of the polymer indicated the presence of a covalent linkage between these segments. Therefore, it was concluded that PhaC(AR) synthesized a novel random-homo block copolymer, P(GL-ran-3HB)-b-P(3HB). |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/80258 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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