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Identifying transcription factors that reduce wood recalcitrance and improve enzymatic degradation of xylem cell wall in Populus
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| Title: | Identifying transcription factors that reduce wood recalcitrance and improve enzymatic degradation of xylem cell wall in Populus |
| Authors: | Hori, Chiaki Browse this author →KAKEN DB | | Takata, Naoki Browse this author | | Lam, Pui Ying Browse this author | | Tobimatsu, Yuki Browse this author | | Nagano, Soichiro Browse this author | | Mortimer, Jenny C. Browse this author | | Cullen, Dan Browse this author |
| Issue Date: | 16-Dec-2020 |
| Publisher: | Nature Research |
| Journal Title: | Scientific reports |
| Volume: | 10 |
| Issue: | 1 |
| Start Page: | 22043 |
| Publisher DOI: | 10.1038/s41598-020-78781-6 |
| Abstract: | Developing an efficient deconstruction step of woody biomass for biorefinery has been drawing considerable attention since its xylem cell walls display highly recalcitrance nature. Here, we explored transcriptional factors (TFs) that reduce wood recalcitrance and improve saccharification efficiency in Populus species. First, 33 TF genes up-regulated during poplar wood formation were selected as potential regulators of xylem cell wall structure. The transgenic hybrid aspens (Populus tremulaxPopulus tremuloides) overexpressing each selected TF gene were screened for in vitro enzymatic saccharification. Of these, four transgenic seedlings overexpressing previously uncharacterized TF genes increased total glucan hydrolysis on average compared to control. The best performing lines overexpressing PtxtERF123 and PtxtZHD14 were further grown to form mature xylem in the greenhouse. Notably, the xylem cell walls exhibited significantly increased total xylan hydrolysis as well as initial hydrolysis rates of glucan. The increased saccharification of PtxtERF123-overexpressing lines could reflect the improved balance of cell wall components, i.e., high cellulose and low xylan and lignin content, which could be caused by upregulation of cellulose synthase genes upon the expression of PtxtERF123. Overall, we successfully identified PtxtERF123 and PtxtZHD14 as effective targets for reducing cell wall recalcitrance and improving the enzymatic degradation of woody plant biomass. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/80405 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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