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TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein |
| Authors: | Kishimoto, Mai Browse this author | | Uemura, Kentaro Browse this author | | Sanaki, Takao Browse this author | | Sato, Akihiko Browse this author | | Hall, William W. Browse this author | | Kariwa, Hiroaki Browse this author →KAKEN DB | | Orba, Yasuko Browse this author →KAKEN DB | | Sawa, Hirofumi Browse this author →KAKEN DB | | Sasaki, Michihito Browse this author →KAKEN DB |
| Keywords: | severe acute respiratory syndrome-like coronavirus-2 (SARS-CoV-2) | | type II transmembrane serine protease (TTSP) | | spike protein |
| Issue Date: | Mar-2021 |
| Publisher: | MDPI |
| Journal Title: | Viruses |
| Volume: | 13 |
| Issue: | 3 |
| Start Page: | 384 |
| Publisher DOI: | 10.3390/v13030384 |
| PMID: | 33671076 |
| Abstract: | Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) utilizes host proteases, including a plasma membrane-associated transmembrane protease, serine 2 (TMPRSS2) to cleave and activate the virus spike protein to facilitate cellular entry. Although TMPRSS2 is a well-characterized type II transmembrane serine protease (TTSP), the role of other TTSPs on the replication of SARS-CoV-2 remains to be elucidated. Here, we have screened 12 TTSPs using human angiotensin-converting enzyme 2-expressing HEK293T (293T-ACE2) cells and Vero E6 cells and demonstrated that exogenous expression of TMPRSS11D and TMPRSS13 enhanced cellular uptake and subsequent replication of SARS-CoV-2. In addition, SARS-CoV-1 and SARS-CoV-2 share the same TTSPs in the viral entry process. Our study demonstrates the impact of host TTSPs on infection of SARS-CoV-2, which may have implications for cell and tissue tropism, for pathogenicity, and potentially for vaccine development. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/80566 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐々木 道仁
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