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Analysis of alpha-Defensin 5 Secretion in Differentiated Caco-2 Cells : Comparison of Cell Bank Origin
| Title: | Analysis of alpha-Defensin 5 Secretion in Differentiated Caco-2 Cells : Comparison of Cell Bank Origin |
| Authors: | Yasuda, Genki Browse this author | | Kobayashi, Masaki Browse this author →KAKEN DB | | Kubota, Atsuhito Browse this author | | Narumi, Katsuya Browse this author →KAKEN DB | | Furugen, Ayako Browse this author →KAKEN DB | | Saito, Yoshitaka Browse this author →KAKEN DB | | Satoh, Takashi Browse this author | | Suzuki, Natsuko Browse this author | | Iseki, Ken Browse this author →KAKEN DB |
| Keywords: | α-defensin 5 | | Caco-2 cell | | cytokine | | differentiation | | alkaline phosphatase |
| Issue Date: | 1-Feb-2021 |
| Publisher: | The Pharmaceutical Society of Japan |
| Journal Title: | Biological and pharmaceutical bulletin |
| Volume: | 44 |
| Issue: | 2 |
| Start Page: | 275 |
| End Page: | 278 |
| Publisher DOI: | 10.1248/bpb.b20-00644 |
| Abstract: | α-Defensin 5 has a particularly broad antibacterial spectrum; it eliminates pathogenic microorganisms
and regulates intestinal flora. Although Caco-2 cells are similar to small intestinal cells, it is unclear
whether they secrete α-defensin 5. Therefore, we investigated whether Caco-2 cells secrete α-defensin 5 and
determined the secretion mechanism using cells from three cell banks (ATCC, DSMZ, and RIKEN). The
Caco-2 cell proliferation rate increased with the number of culture days, irrespective of cell bank origin.
On the other hand, the alkaline phosphatase activity, which affects cell differentiation and the mRNA levels
of several cytokines, such as interleukin 8 (IL-8), IL-6, IL-1β, tumor necrosis factor-α (TNF-α), and IL-2,
in the Caco-2 cells fluctuated with the number of culture days, and differed for each cell bank. α-Defensin
5 secretion was detected in all three cell bank Caco-2 cells; particularly, the ATCC Caco-2 cells grew linearly
depending on the cell culture day as well as the levels of IL-8 and TNF-α mRNA. This suggested that
α-defensin 5 secretion in the ATCC Caco-2 cells was associated with fluctuations in the mRNA levels of
various cytokines, such as IL-8 and TNF-α. In conclusion, Caco-2 cells may be a simple model for screening
health food components and drugs that affect α-defensin 5 secretion. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81034 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 小林 正紀
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