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Analysis of alpha-Defensin 5 Secretion in Differentiated Caco-2 Cells : Comparison of Cell Bank Origin

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Title: Analysis of alpha-Defensin 5 Secretion in Differentiated Caco-2 Cells : Comparison of Cell Bank Origin
Authors: Yasuda, Genki Browse this author
Kobayashi, Masaki Browse this author →KAKEN DB
Kubota, Atsuhito Browse this author
Narumi, Katsuya Browse this author →KAKEN DB
Furugen, Ayako Browse this author →KAKEN DB
Saito, Yoshitaka Browse this author →KAKEN DB
Satoh, Takashi Browse this author
Suzuki, Natsuko Browse this author
Iseki, Ken Browse this author →KAKEN DB
Keywords: α-defensin 5
Caco-2 cell
cytokine
differentiation
alkaline phosphatase
Issue Date: Feb-2021
Publisher: The Pharmaceutical Society of Japan
Journal Title: Biological and pharmaceutical bulletin
Volume: 44
Issue: 2
Start Page: 275
End Page: 278
Publisher DOI: 10.1248/bpb.b20-00644
Abstract: α-Defensin 5 has a particularly broad antibacterial spectrum; it eliminates pathogenic microorganisms and regulates intestinal flora. Although Caco-2 cells are similar to small intestinal cells, it is unclear whether they secrete α-defensin 5. Therefore, we investigated whether Caco-2 cells secrete α-defensin 5 and determined the secretion mechanism using cells from three cell banks (ATCC, DSMZ, and RIKEN). The Caco-2 cell proliferation rate increased with the number of culture days, irrespective of cell bank origin. On the other hand, the alkaline phosphatase activity, which affects cell differentiation and the mRNA levels of several cytokines, such as interleukin 8 (IL-8), IL-6, IL-1β, tumor necrosis factor-α (TNF-α), and IL-2, in the Caco-2 cells fluctuated with the number of culture days, and differed for each cell bank. α-Defensin 5 secretion was detected in all three cell bank Caco-2 cells; particularly, the ATCC Caco-2 cells grew linearly depending on the cell culture day as well as the levels of IL-8 and TNF-α mRNA. This suggested that α-defensin 5 secretion in the ATCC Caco-2 cells was associated with fluctuations in the mRNA levels of various cytokines, such as IL-8 and TNF-α. In conclusion, Caco-2 cells may be a simple model for screening health food components and drugs that affect α-defensin 5 secretion.
Type: article
URI: http://hdl.handle.net/2115/81034
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 正紀

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