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In vitro simultaneous mapping of the partial pressure of oxygen, pH and inorganic phosphate using electron paramagnetic resonance

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Title: In vitro simultaneous mapping of the partial pressure of oxygen, pH and inorganic phosphate using electron paramagnetic resonance
Authors: Taguchi, Akihiro Browse this author
DeVience, Stephen Browse this author
Driesschaert, Benoit Browse this author
Khramtsov, Valery V. Browse this author
Hirata, Hiroshi Browse this author →KAKEN DB
Issue Date: 7-May-2020
Publisher: Royal Society of Chemistry
Journal Title: Analyst
Volume: 145
Issue: 9
Start Page: 3236
End Page: 3244
Publisher DOI: 10.1039/d0an00168f
Abstract: The partial pressure of oxygen (pO(2)) and the extracellular pH in the tumour microenvironment are essential parameters for understanding the physiological state of a solid tumour. Also, phosphate-containing metabolites are involved in energy metabolism, and interstitial inorganic phosphate (Pi) is an informative marker for tumour growth. This article describes the simultaneous mapping of pO(2), pH and Pi using 750 MHz continuous-wave (CW) electron paramagnetic resonance (EPR) and a multifunctional probe, monophosphonated trityl radical p(1)TAM-D. The concept was demonstrated by acquiring three-dimensional (3D) maps of pO(2), pH and Pi for multiple solution samples. This was made possible by combining a multifunctional radical probe, fast CW-EPR spectral acquisition, four-dimensional (4D) spectral-spatial image reconstruction, and spectral fitting. The experimental results of mapping pO(2), pH and Pi suggest that the concept of simultaneous mapping using EPR is potentially applicable for the multifunctional measurements of a mouse tumour model.
Type: article (author version)
URI: http://hdl.handle.net/2115/81120
Appears in Collections:情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 平田 拓

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