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Spatiotemporal dynamics of single cell stiffness in the early developing ascidian chordate embryo

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Title: Spatiotemporal dynamics of single cell stiffness in the early developing ascidian chordate embryo
Authors: Fujii, Yuki Browse this author
Koizumi, Wataru C. Browse this author
Imai, Taichi Browse this author
Yokobori, Megumi Browse this author
Matsuo, Tomohiro Browse this author
Oka, Kotaro Browse this author
Hotta, Kohji Browse this author
Okajima, Takaharu Browse this author →KAKEN DB
Issue Date: 16-Mar-2021
Publisher: Nature Research
Journal Title: Communications biology
Volume: 4
Issue: 1
Start Page: 341
Publisher DOI: 10.1038/s42003-021-01869-w
Abstract: During the developmental processes of embryos, cells undergo massive deformation and division that are regulated by mechanical cues. However, little is known about how embryonic cells change their mechanical properties during different cleavage stages. Here, using atomic force microscopy, we investigated the stiffness of cells in ascidian embryos from the fertilised egg to the stage before gastrulation. In both animal and vegetal hemispheres, we observed a Rho kinase (ROCK)-independent cell stiffening that the cell stiffness exhibited a remarkable increase at the timing of cell division where cortical actin filaments were organized. Furthermore, in the vegetal hemisphere, we observed another mechanical behaviour, i.e., a ROCK-associated cell stiffening, which was retained even after cell division or occurred without division and propagated sequentially toward adjacent cells, displaying a characteristic cell-to-cell mechanical variation. The results indicate that the mechanical properties of embryonic cells are regulated at the single cell level in different germ layers. Fujii et al. investigate the stiffness of cells in ascidian embryos from the fertilised egg to the stage before gastrulation. They find two types of cell stiffening, occurring during cell division and in the interphase, the latter of which is associated with the Rho kinase pathway. They conclude that the mechanical properties of early embryonic cells are regulated specifically at the single cell level.
Type: article
URI: http://hdl.handle.net/2115/81235
Appears in Collections:情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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