Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Information Science and Technology / Faculty of Information Science and Technology >
Peer-reviewed Journal Articles, etc >
Spatiotemporal dynamics of single cell stiffness in the early developing ascidian chordate embryo
Title: | Spatiotemporal dynamics of single cell stiffness in the early developing ascidian chordate embryo |
Authors: | Fujii, Yuki Browse this author | Koizumi, Wataru C. Browse this author | Imai, Taichi Browse this author | Yokobori, Megumi Browse this author | Matsuo, Tomohiro Browse this author | Oka, Kotaro Browse this author | Hotta, Kohji Browse this author | Okajima, Takaharu Browse this author →KAKEN DB |
Issue Date: | 16-Mar-2021 |
Publisher: | Nature Research |
Journal Title: | Communications biology |
Volume: | 4 |
Issue: | 1 |
Start Page: | 341 |
Publisher DOI: | 10.1038/s42003-021-01869-w |
Abstract: | During the developmental processes of embryos, cells undergo massive deformation and division that are regulated by mechanical cues. However, little is known about how embryonic cells change their mechanical properties during different cleavage stages. Here, using atomic force microscopy, we investigated the stiffness of cells in ascidian embryos from the fertilised egg to the stage before gastrulation. In both animal and vegetal hemispheres, we observed a Rho kinase (ROCK)-independent cell stiffening that the cell stiffness exhibited a remarkable increase at the timing of cell division where cortical actin filaments were organized. Furthermore, in the vegetal hemisphere, we observed another mechanical behaviour, i.e., a ROCK-associated cell stiffening, which was retained even after cell division or occurred without division and propagated sequentially toward adjacent cells, displaying a characteristic cell-to-cell mechanical variation. The results indicate that the mechanical properties of embryonic cells are regulated at the single cell level in different germ layers. Fujii et al. investigate the stiffness of cells in ascidian embryos from the fertilised egg to the stage before gastrulation. They find two types of cell stiffening, occurring during cell division and in the interphase, the latter of which is associated with the Rho kinase pathway. They conclude that the mechanical properties of early embryonic cells are regulated specifically at the single cell level. |
Type: | article |
URI: | http://hdl.handle.net/2115/81235 |
Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|