| Title: | Hyperpolarized C-13 Magnetic Resonance Imaging of Fumarate Metabolism by Parahydrogen-induced Polarization: A Proof-of-Concept in vivo Study |
| Authors: | Stewart, Neil J. Browse this author |
| Nakano, Hitomi Browse this author |
| Sugai, Shuto Browse this author |
| Tomohiro, Mitsushi Browse this author |
| Kase, Yuki Browse this author |
| Uchio, Yoshiki Browse this author |
| Yamaguchi, Toru Browse this author |
| Matsuo, Yujirou Browse this author |
| Naganuma, Tatsuya Browse this author |
| Takeda, Norihiko Browse this author |
| Nishimura, Ikuya Browse this author →KAKEN DB |
| Hirata, Hiroshi Browse this author →KAKEN DB |
| Hashimoto, Takuya Browse this author |
| Matsumoto, Shingo Browse this author →KAKEN DB |
| Keywords: | density functional calculations |
| fumarate |
| hyperpolarized C-13 MRS/I |
| matabolism |
| parahydrogen-induced polarization |
| Issue Date: | 17-May-2021 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | ChemPhysChem |
| Volume: | 22 |
| Issue: | 10 |
| Start Page: | 915 |
| End Page: | 923 |
| Publisher DOI: | 10.1002/cphc.202001038 |
| Abstract: | Hyperpolarized [1-C-13]fumarate is a promising magnetic resonance imaging (MRI) biomarker for cellular necrosis, which plays an important role in various disease and cancerous pathological processes. To demonstrate the feasibility of MRI of [1-C-13]fumarate metabolism using parahydrogen-induced polarization (PHIP), a low-cost alternative to dissolution dynamic nuclear polarization (dDNP), a cost-effective and high-yield synthetic pathway of hydrogenation precursor [1-C-13]acetylenedicarboxylate (ADC) was developed. The trans-selectivity of the hydrogenation reaction of ADC using a ruthenium-based catalyst was elucidated employing density functional theory (DFT) simulations. A simple PHIP set-up was used to generate hyperpolarized [1-C-13]fumarate at sufficient C-13 polarization for ex vivo detection of hyperpolarized C-13 malate metabolized from fumarate in murine liver tissue homogenates, and in vivo C-13 MR spectroscopy and imaging in a murine model of acetaminophen-induced hepatitis. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/81397 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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