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Simple enumeration of Escherichia coli concentrations in river water samples by measuring beta-d-glucuronidase activities in a microplate reader

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/81524

Title: Simple enumeration of Escherichia coli concentrations in river water samples by measuring beta-d-glucuronidase activities in a microplate reader
Authors: Satoh, Hisashi Browse this author →KAKEN DB
Katayose, Yutaka Browse this author
Hirano, Reiko Browse this author
Keywords: enzyme
fecal pollution
fluctuation
fluorescence
Sapporo
wastewater
Issue Date: 15-Mar-2021
Publisher: IWA Publishing
Journal Title: Water Science and Technology
Volume: 83
Issue: 6
Start Page: 1399
End Page: 1406
Publisher DOI: 10.2166/wst.2021.072
Abstract: Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory to enumerate E. coli concentrations in RW samples. The method is based on the use of the synthetic substrate specific for the beta-d-glucuronidase (GUS) produced by E. coli. GUS activities and E. coli concentrations were monitored at eight selected sites in rivers running through Sapporo, Japan. Because the fluorescence intensities of the synthetic substrate in the RW samples increased linearly over a 4-h incubation period, we could estimate the GUS activities of the RW samples. The GUS activities were highly correlated with E. coli concentrations at >100 most probable numbers 100 mL(-1) with a correlation coefficient of 0.87. The GUS activities of the RW samples collected from all sampling sites fitted well to a single correlation equation, which indicates that it was applicable to the estimation of E. coli concentrations regardless of the sampling sites. This method is simple, rapid, reliable, inexpensive, and high throughput, and is therefore useful for monitoring E. coli in RW.
Type: article (author version)
URI: http://hdl.handle.net/2115/81524
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐藤 久

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