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Simple enumeration of Escherichia coli concentrations in river water samples by measuring beta-d-glucuronidase activities in a microplate reader
Title: | Simple enumeration of Escherichia coli concentrations in river water samples by measuring beta-d-glucuronidase activities in a microplate reader |
Authors: | Satoh, Hisashi Browse this author →KAKEN DB | Katayose, Yutaka Browse this author | Hirano, Reiko Browse this author |
Keywords: | enzyme | fecal pollution | fluctuation | fluorescence | Sapporo | wastewater |
Issue Date: | 15-Mar-2021 |
Publisher: | IWA Publishing |
Journal Title: | Water Science and Technology |
Volume: | 83 |
Issue: | 6 |
Start Page: | 1399 |
End Page: | 1406 |
Publisher DOI: | 10.2166/wst.2021.072 |
Abstract: | Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory to enumerate E. coli concentrations in RW samples. The method is based on the use of the synthetic substrate specific for the beta-d-glucuronidase (GUS) produced by E. coli. GUS activities and E. coli concentrations were monitored at eight selected sites in rivers running through Sapporo, Japan. Because the fluorescence intensities of the synthetic substrate in the RW samples increased linearly over a 4-h incubation period, we could estimate the GUS activities of the RW samples. The GUS activities were highly correlated with E. coli concentrations at >100 most probable numbers 100 mL(-1) with a correlation coefficient of 0.87. The GUS activities of the RW samples collected from all sampling sites fitted well to a single correlation equation, which indicates that it was applicable to the estimation of E. coli concentrations regardless of the sampling sites. This method is simple, rapid, reliable, inexpensive, and high throughput, and is therefore useful for monitoring E. coli in RW. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/81524 |
Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐藤 久
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