HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >

Molecular phylogeny and ultrastructure of two novel parasitic dinoflagellates,Haplozoon gracile sp. nov. and H. pugnus sp. nov.

Files in This Item:
Phycologia 59-4_305-319.pdf8.99 MBPDFView/Open
Supp Vid 1.mp4885.62 kBMP4 videoView/Open
Supp Vid 2.mp46.56 MBMP4 videoView/Open
Please use this identifier to cite or link to this item:

Title: Molecular phylogeny and ultrastructure of two novel parasitic dinoflagellates,Haplozoon gracile sp. nov. and H. pugnus sp. nov.
Authors: Yamamoto, Mana Browse this author
Wakeman, Kevin C. Browse this author
Tomioka, Shinri Browse this author
Horiguchi, Takeo Browse this author →KAKEN DB
Keywords: Alveolata
Bamboo worms
Issue Date: Jul-2020
Publisher: Taylor & Francis
Journal Title: Phycologia
Volume: 59
Issue: 4
Start Page: 305
End Page: 319
Publisher DOI: 10.1080/00318884.2020.1753427
Abstract: This study describes two novel parasitic dinoflagellates:Haplozoon gracile sp. nov. isolated from a bamboo worm (Maldanidae), 'cf.Petaloclymenesp.'sensuKobayashiet al. 2018; and,H. pugnus sp. nov. isolated fromNicomachesp. andNicomache personata(Maldanidae). Trophonts (feeding stages) were observed with light, scanning, and transmission electron microscopy. Molecular phylogenetic analyses were performed based on 18S rDNA. COI sequences were obtained for host organisms. Trophonts ofH. gracilewere linear (single longitudinal row) and relatively slender with a mean length of 190 mu m, and consisted of a long and narrow trophocyte, rectangular gonocytes (mean width = 10 mu m), and slightly rounded sporocytes. Trophonts ofH. pugnuswere pectinate (1-8 rows of sporocytes in one plane), with a mean length of 179 mu m, consisting of a bulbous trophocyte, rectangular gonocytes (mean width = 25 mu m), and rounded sporocytes. The body of both species was covered with many depressions that overlaid the amphiesmal vesicles. TEM observations of trophocytes inH. gracilerevealed a stylet with a central dense core and rich mitochondria subtending the amphiesma. Furthermore, amphiesmal vesicles appeared to contain thecal plates in both species. Phylogenetic analyses generally resolved aHaplozoonclade, andH. gracileandH. pugnuswere clearly distinguished from other species for which molecular data are available. Based on the morphological and host comparisons with all described species and their molecular phylogeny, we conclude that these two isolates are new species ofHaplozoon, H. gracile sp. nov. andH. pugnus sp. nov.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Phycologia on Published online: 08 Jun 2020, available online:
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 堀口 健雄

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University