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Hokkaido University Collection of Scholarly and Academic Papers >
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High Production of Ergothioneine in Escherichia coli using the Sulfoxide Synthase from Methylobacterium strains
| Title: | High Production of Ergothioneine in Escherichia coli using the Sulfoxide Synthase from Methylobacterium strains |
| Authors: | Kamide, Tomoyuki Browse this author | | Takusagawa, Shun Browse this author | | Tanaka, Naoyuki Browse this author | | Ogasawara, Yasushi Browse this author | | Kawano, Yusuke Browse this author | | Ohtsu, Iwao Browse this author | | Satoh, Yasuharu Browse this author →KAKEN DB | | Dairi, Tohru Browse this author →KAKEN DB |
| Keywords: | ergothioneine | | heterologous production | | Escherichia coli | | Methylobacterium |
| Issue Date: | 10-Jun-2020 |
| Publisher: | American Chemical Society |
| Journal Title: | Journal of Agricultural and Food Chemistry |
| Volume: | 68 |
| Issue: | 23 |
| Start Page: | 6390 |
| End Page: | 6394 |
| Publisher DOI: | 10.1021/acs.jafc.0c01846 |
| Abstract: | We previously constructed a heterologous production system for ergothioneine (ERG) in Escherichia coli using five ERG biosynthesis genes (egtABCDE) from Mycobacterium smegmatis. However, significant amounts of hercynine (HER), an intermediate of ERG, as ERG were accumulated, suggesting that the reaction of EgtB catalyzing the attachment of gamma-glutamylcysteine (gamma GC) to HER to yield hercynyl-gamma-glutamylcysteine sulfoxide was a bottleneck. In this study, we searched for other EgtBs and found many egtB orthologs in diverse microorganisms. Among these, Methylobacterium strains possessed EgtBs that catalyze the direct conversion of HER into hercynylcysteine sulfoxide with L-cysteine (L-Cys) as a sulfur donor, in a manner similar to those of acidobacterial CthEgtB and fungal Egt1. An in vitro study with recombinant EgtBs from Methylobacterium brachiatum and Methylobacterium pseudosasicola clearly showed that both enzymes accepted L-Cys but not gamma GC. We reconstituted the ERG production system in E. coli with egtB from M. pseudosasicola; ERG productivity reached 657 mg L-1. |
| Rights: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jafc.0c01846, see http://pubs.acs.org/page/policy/articlesonrequest/index.html. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/81773 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐藤 康治
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