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Bio-Mediated Soil Improvement Using Plant Derived Enzyme in Addition to Magnesium Ion

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Title: Bio-Mediated Soil Improvement Using Plant Derived Enzyme in Addition to Magnesium Ion
Authors: Imran, Md Al Browse this author
Nakashima, Kazunori Browse this author
Kawasaki, Satoru Browse this author →KAKEN DB
Keywords: enzyme-induced carbonate precipitation (EICP)
urease activity
Ca2+ ratios
watermelon seeds
CaCO3 morphology
soil improvement
plant-derived urease enzyme
Issue Date: May-2021
Publisher: MDPI
Journal Title: Crystals
Volume: 11
Issue: 5
Start Page: 516
Publisher DOI: 10.3390/cryst11050516
Abstract: Recently, soil improvement using EICP (Enzyme-Induced Carbonate Precipitation) methods in the geotechnical and geo-environmental field has become a prominent interest worldwide. The objective of this study was to develop an improved extraction technique of crude urease from watermelon seeds in both dry and germinated conditions. Subsequently, this study also analyzed the improvement methodology of crystal polymorphs and soil bonding incorporation of various Mg2+/Ca2+ ratios. The optimization of enzyme-mediated carbonate precipitation was also investigated by Scanning Electron Microscope (SEM) and X-ray Diffraction (XRD) analysis. Results confirmed that the precipitated crystals are mainly calcite, vaterite and aragonite primarily (depending on the Mg2+/Ca2+ ratios). Therefore, to improve the bonding capacity in between the sand particles a novel improvement methodology was investigated by adding various Mg2+/Ca2+ ratios. The mechanical properties of the treated soil (Mikawa Sand, D-50 = 0.870 mm) specimens were tested by unconfined compressive strength (UCS) and this confirmed the effectiveness of adding various Mg2+/Ca2+ ratios. The results of the UCS tests showed that, the lower molar ratios of Mg2+/Ca2+ can significantly improve the UCS of the specimen (up to 50%) which could be considered a significant outcome for different bio-geotechnical applications.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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