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Simple Approach for Fluorescence Signal Amplification Utilizing a Poly(vinyl alcohol)-Based Polymer Structure in a Microchannel
| Title: | Simple Approach for Fluorescence Signal Amplification Utilizing a Poly(vinyl alcohol)-Based Polymer Structure in a Microchannel |
| Authors: | Nishiyama, Keine Browse this author | | Maeki, Masatoshi Browse this author | | Ishida, Akihiko Browse this author | | Tani, Hirofumi Browse this author →KAKEN DB | | Hisamoto, Hideaki Browse this author | | Tokeshi, Manabu Browse this author →KAKEN DB |
| Keywords: | Mixtures | | Chemical structure | | Fluorescence | | Molecules | | Noncovalent probes |
| Issue Date: | 30-Mar-2021 |
| Publisher: | American Chemical Society |
| Journal Title: | ACS Omega |
| Volume: | 6 |
| Issue: | 12 |
| Start Page: | 8340 |
| End Page: | 8345 |
| Publisher DOI: | 10.1021/acsomega.1c00057 |
| Abstract: | Analytical methods with fluorescence detection are in widespread use for detecting low abundance analytes. Here, we report a simple method for fluorescence signal amplification utilizing a structure of an azide-unit pendant water-soluble photopolymer (AWP) in a microchannel. The AWP is a poly(vinyl alcohol)-based photocross-linkable polymer, which is often used in biosensors. We determined that the wall-like structure of the AWP (AWP-wall) constructed in a microchannel functioned as an amplifier of a fluorescence signal. When a solution of fluorescent molecules was introduced into the microchannel having the AWP-wall, the fluorescent molecules accumulated inside the AWP-wall by diffusion. Consequently, the fluorescence intensity inside the AWP-wall increased locally. Among the fluorescent molecules considered in this paper, 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) (DDAO) showed the highest efficiency of fluorescence signal amplification. We prepared a calibration curve for DDAO using the fluorescence intensity inside the AWP-wall, and the sensitivity was 5-fold that for the microchannel without the AWP-wall. This method realizes the improved sensitivity of fluorescence detection easily because the fluorescence signal was amplified only by injecting the solution into the microchannel having the AWP-wall. Furthermore, since this method is not limited to only the use of microchannel, we expect it to be applicable in various fields. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82056 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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