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The mechanism of Tyk2 deficiency-induced immunosuppression in mice involves robust IL-10 production in macrophages

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Title: The mechanism of Tyk2 deficiency-induced immunosuppression in mice involves robust IL-10 production in macrophages
Authors: Hirashima, Koki Browse this author
Muromoto, Ryuta Browse this author →KAKEN DB
Minoguchi, Hiroya Browse this author
Matsumoto, Tomohiro Browse this author
Kitai, Yuichi Browse this author →KAKEN DB
Kashiwakura, Jun-Ichi Browse this author →KAKEN DB
Shimoda, Kazuya Browse this author
Oritani, Kenji Browse this author
Matsuda, Tadashi Browse this author →KAKEN DB
Keywords: Tyk2
Macrophage plasticity
Issue Date: Jun-2020
Publisher: Elsevier
Journal Title: Cytokine
Volume: 130
Start Page: 155077
Publisher DOI: 10.1016/j.cyto.2020.155077
Abstract: Macrophages are highly plastic in their pro-inflammatory/anti-inflammatory roles. Type I and II interferons (IFNs) are known to modulate macrophage activation. Tyrosine kinase 2 (Tyk2) has an intimate relationship with type I and II IFN signaling. Animal studies have shown that Tyk2 knock-out (KO) in mice is associated with reduced inflammatory responses in various mouse models of diseases. To investigate the role of Tyk2 in inflammation in more detail, we intraperitoneally injected heat-killed Propionibacterium acnes (P. acnes) to Tyk2 KO mice. P. acnes-induced acute peritoneal inflammation, assessed by neutrophil infiltration, was reduced in Tyk2 KO mice. The reduction was accompanied with diminished productions of inflammatory cytokines and an enhanced production of anti-inflammatory IL-10. Unexpectedly, pre-treatment of wild-type mice with the neutralizing antibodies for IFNs did not affect P. acnes-induced neutrophil infiltration. A neutralizing antibody for the IL-10 receptor in Tyk2 KO mice restored P. acnes-induced peritoneal inflammation. Enhanced production of IL-10 from Tyk2 KO peritoneal cells was suppressed by either the cyclooxygenase inhibitor diclofenac or protein kinase A inhibitor H-89. The level of prostaglandin E-2 (PGE(2)) in the steady-state peritoneal cavity in Tyk2 KO mice was higher than that in wild-type mice. Tyk2 KO macrophages showed an enhanced CREB phosphorylation induced by P. acnes plus PGE(2). Taken together, these results showed that Tyk2 deficiency potentiates the PGE(2)-protein kinase A-IL-10 pathway in macrophages, and thereby contributes to potentiation of the immunosuppressive phenotype.
Rights: ©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 室本 竜太

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