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Follicle priming by FSH and pre-maturation culture to improve oocyte quality in vivo and in vitro

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Title: Follicle priming by FSH and pre-maturation culture to improve oocyte quality in vivo and in vitro
Authors: Sakaguchi, Kenichiro Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Keywords: FSH
Ovum pick-up
In vivo maturation
Single injection
In vitro pre-maturation
Issue Date: 1-Jul-2020
Publisher: Elsevier
Journal Title: Theriogenology
Volume: 150
Start Page: 122
End Page: 129
Publisher DOI: 10.1016/j.theriogenology.2020.01.023
Abstract: Nowadays there is strong demand to produce embryos from premium quality cattle, and we can produce embryos using oocytes collected from living premium animals by ovum-pick up (OPU) followed by in vitro fertilization (IVF). However, the developmental competence of IVF oocytes to form blastocysts is variable. The developmental competence of oocytes depends on the size and stages of follicles, and follicle-stimulating hormone priming (FSH-priming) prior to OPU can promote follicular growth and improve the developmental competence of oocytes. Furthermore, following the induction of ovulation using an injection of luteinizing hormone or gonadotropin-releasing hormone after FSH-priming, we can collect in vivo matured oocytes from ovulatory follicles, which show higher developmental competence than oocytes matured in vitro. However, the conventional protocols for FSH-priming consist of multiple FSH injection for 3-4 days, which is stressful for the animal and labor-intensive for the veterinarian. In addition, these techniques cannot be applied to IVF of oocytes collected from bovine ovaries derived from slaughterhouses, which are important sources of oocytes. Here, we review previous research focused on FSH-priming, especially for collecting in vivo matured oocytes and a simplified method for super-stimulation using a single injection of FSH. We also introduce the previous achievements using in vitro pre-maturation culture, which can improve the developmental competence of oocytes derived from non-stimulated animals. (C) 2020 Elsevier Inc. All rights reserved.
Rights: © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 坂口 謙一郎

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