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Diverse meibum lipids produced by Awat1 and Awat2 are important for stabilizing tear film and protecting the ocular surface
| Title: | Diverse meibum lipids produced by Awat1 and Awat2 are important for stabilizing tear film and protecting the ocular surface |
| Authors: | Sawai, Megumi Browse this author | | Watanabe, Keisuke Browse this author | | Tanaka, Kana Browse this author | | Kinoshita, Wataru Browse this author | | Otsuka, Kento Browse this author | | Miyamoto, Masatoshi Browse this author | | Sassa, Takayuki Browse this author →KAKEN DB | | Kihara, Akio Browse this author →KAKEN DB |
| Issue Date: | 21-May-2021 |
| Publisher: | Cell Press |
| Journal Title: | iScience |
| Volume: | 24 |
| Issue: | 5 |
| Start Page: | 102478 |
| Publisher DOI: | 10.1016/j.isci.2021.102478 |
| Abstract: | A lipid layer consisting of meibum lipids exists in the tear filmand functions in preventing dry eye disease. Although the meibum lipids include diverse lipid classes, the synthesis pathway and role of each class remain largely unknown. Here, we created single and double knockout (KO and DKO, respectively) mice for the two acyl-CoA wax alcohol acyltransferases (Awat1 and Awat2) and investigated their dry eye phenotypes and meibum lipid composition. Awat2 KO and DKO mice exhibited severe dry eye with meibomian gland dysfunction, whereas Awat1 KO mice had mild dry eye. In these mice, specific meibum lipid classes were reduced: (O-acyl)-omega-hydroxy fatty acids and type 1u wax diesters in Awat1 KO mice, wax monoesters and types 1u and 2u wax diesters in Awat2 KO mice, and most of these in DKO mice. Our findings reveal that Awat1 and Awat2 show characteristic substrate specificity and together produce diverse meibum lipids. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82244 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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