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Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives

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Title: Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives
Authors: Iwata, Mana Browse this author
Gutierrez, Ana Browse this author
Marques, Gisela Browse this author
Sabat, Grzegorz Browse this author
Kersten, Philip J. Browse this author
Cullen, Daniel Browse this author
Bhatnagar, Jennifer M. Browse this author
Yadav, Jagjit Browse this author
Lipzen, Anna Browse this author
Yoshinaga, Yuko Browse this author
Sharma, Aditi Browse this author
Adam, Catherine Browse this author
Daum, Christopher Browse this author
Ng, Vivian Browse this author
Grigoriev, Igor V. Browse this author
Hori, Chiaki Browse this author →KAKEN DB
Issue Date: 15-Jun-2021
Publisher: Nature Research
Journal Title: Scientific reports
Volume: 11
Issue: 1
Start Page: 12528
Publisher DOI: 10.1038/s41598-021-91756-5
Abstract: Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efficiently transform or degrade conifer extractives but little is known about the mechanism(s). In this study, to clarify the mechanism(s) of softwood degradation, we examined the transcriptome, proteome, and metabolome of P. gigantea when grown on defined media containing microcrystalline cellulose and pine sapwood extractives. Beyond the conventional enzymes often associated with cellulose, hemicellulose and lignin degradation, an array of enzymes implicated in the metabolism of softwood lipophilic extractives such as fatty and resin acids, steroids and glycerides was significantly up-regulated. Among these, a highly expressed and inducible lipase is likely responsible for lipophilic extractive degradation, based on its extracellular location and our characterization of the recombinant enzyme. Our results provide insight into physiological roles of extractives in the interaction between wood and fungi.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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