| Title: | Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives |
| Authors: | Iwata, Mana Browse this author |
| Gutierrez, Ana Browse this author |
| Marques, Gisela Browse this author |
| Sabat, Grzegorz Browse this author |
| Kersten, Philip J. Browse this author |
| Cullen, Daniel Browse this author |
| Bhatnagar, Jennifer M. Browse this author |
| Yadav, Jagjit Browse this author |
| Lipzen, Anna Browse this author |
| Yoshinaga, Yuko Browse this author |
| Sharma, Aditi Browse this author |
| Adam, Catherine Browse this author |
| Daum, Christopher Browse this author |
| Ng, Vivian Browse this author |
| Grigoriev, Igor V. Browse this author |
| Hori, Chiaki Browse this author →KAKEN DB |
| Issue Date: | 15-Jun-2021 |
| Publisher: | Nature Research |
| Journal Title: | Scientific reports |
| Volume: | 11 |
| Issue: | 1 |
| Start Page: | 12528 |
| Publisher DOI: | 10.1038/s41598-021-91756-5 |
| Abstract: | Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efficiently transform or degrade conifer extractives but little is known about the mechanism(s). In this study, to clarify the mechanism(s) of softwood degradation, we examined the transcriptome, proteome, and metabolome of P. gigantea when grown on defined media containing microcrystalline cellulose and pine sapwood extractives. Beyond the conventional enzymes often associated with cellulose, hemicellulose and lignin degradation, an array of enzymes implicated in the metabolism of softwood lipophilic extractives such as fatty and resin acids, steroids and glycerides was significantly up-regulated. Among these, a highly expressed and inducible lipase is likely responsible for lipophilic extractive degradation, based on its extracellular location and our characterization of the recombinant enzyme. Our results provide insight into physiological roles of extractives in the interaction between wood and fungi. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82379 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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