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Rapid and Simple Detection of Isoniazid-Resistant Mycobacterium tuberculosis Utilizing a DNA Chromatography-Based Technique

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Title: Rapid and Simple Detection of Isoniazid-Resistant Mycobacterium tuberculosis Utilizing a DNA Chromatography-Based Technique
Authors: Kodera, Takuya Browse this author
Yamaguchi, Tomoyuki Browse this author
Fukushima, Yukari Browse this author
Kobayashi, Kumi Browse this author
Takarada, Yutaka Browse this author
Chizimu, Joseph Yamweka Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Solo, Eddie Samuneti Browse this author
Lungu, Patrick Saili Browse this author
Kawase, Mitsuo Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Issue Date: May-2021
Publisher: 国立感染症研究所(National Institute of Infectious Diseases, Japan)
Journal Title: Japanese journal of infectious diseases
Volume: 74
Issue: 3
Start Page: 214
End Page: 219
Publisher DOI: 10.7883/yoken.JJID.2020.754
Abstract: Despite the availability of anti-tuberculosis drugs, the treatment of tuberculosis has been complicated by drug-resistant tuberculosis. The early detection of drug resistance makes early treatment possible. However. the available tools are mainly for rifampicin resistance detection, and the existing isoniazid resistance detection method is expensive, highly technical, and complicated, making it unsustainable for use in developing nations. This study aimed to develop a simple, rapid, and low-cost diagnostic kit for isoniazid-resistant tuberculosis using the single-stranded tag hybridization method to target an isoniazid resistance-conferring mutation. Specificity and sensitivity were assessed using DNA extracted from 49 isoniazid-resistant and 41 isoniazid-susceptible Mycobacterium tuberculosis clinical isolates cultured in mycobacterial growth indicator tubes. Positive signals were observed on mutant and wild-type lines with 100% sensitivity and specificity compared with Sanger sequencing results. In contrast, no positive signal was observed for non-tuberculosis mycobacteria. The detection limit of this method was 10(3) CFU or less. The STH-PAS system for isoniazid-resistant M. tuberculosis detection developed in this study offers a better alternative to conventional phenotypic isoniazid resistance determination, which will be of both clinical and epidemiological significance in resource-limited nations.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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