| Title: | Pharmacological Alteration of Cellular Mechanical Properties in Pulmonary Arterial Smooth Muscle Cells of Idiopathic Pulmonary Arterial Hypertension |
| Authors: | Katsuragi, Shinichi Browse this author →KAKEN DB |
| Tatsumi, Nao Browse this author |
| Matsumoto, Mizuki Browse this author |
| Narita, Jun Browse this author →KAKEN DB |
| Ishii, Ryo Browse this author |
| Suginobe, Hidehiro Browse this author →KAKEN DB |
| Tsuru, Hirofumi Browse this author |
| Wang, Renjie Browse this author |
| Kogaki, Shigetoyo Browse this author →KAKEN DB |
| Tanaka, Ryosuke Browse this author |
| Ozono, Keiichi Browse this author →KAKEN DB |
| Okajima, Takaharu Browse this author →KAKEN DB |
| Ishida, Hidekazu Browse this author →KAKEN DB |
| Keywords: | Pulmonary arterial hypertension |
| Atomic force microscopy |
| Smooth muscle cells |
| Cellular mechanical properties |
| Elasticity |
| Fluidity |
| Issue Date: | Aug-2021 |
| Publisher: | Elmer Press |
| Journal Title: | Cardiology Research |
| Volume: | 12 |
| Issue: | 4 |
| Start Page: | 231 |
| End Page: | 237 |
| Publisher DOI: | 10.14740/cr1282 |
| Abstract: | Background: Idiopathic pulmonary arterial hypertension (IPAH) is a progressive disease caused by vascular remodeling of the pulmonary arteries with elevated pulmonary vascular resistance. Recently, various pulmonary vasodilator drugs have become available in the clinical field, and have dramatically ameliorated the prognosis of IPAH. However, little is known about how the mechanical properties of pulmonary arterial smooth muscle cells (PASMCs) are altered under drug supplementation. Methods: Atomic force microscopy (AFM) was used to investigate the mechanical properties of PASMCs derived from a patient with IPAH (PAH-PASMCs) and a healthy control (N-PASMCs) which received the supplementation of clinically used drugs for IPAH: sildenafil, macitentan, and riociguat. Results: PASMCs derived from PAH-PASMCs were stiffer than those derived from N-PASMCs. With sildenafil treatment, the apparent Young's modulus (E-0) of cells significantly decreased in PAH-PASMCs but remained unchanged in N-PASMCs. The decrease in E-0 of PAH-PASMCs was also observed in macitentan and riociguat treatment. The stress relaxation AFM revealed that the decrease in E-0 of PAH-PASMCs resulted from a decrease in the cell elastic modulus and/or increase in cell fluidity. The combination treatment of macitentan and riociguat showed an additive effect on cell mechanical properties, implying that this clinically accepted combination therapy for IPAH influences the intracellular mechanical components. Conclusions: Pulmonary vasodilator drugs affect the mechanical properties of PAH-PASMCs, and there exists a mechanical effect of combination treatment on PAH-PASMCs. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82468 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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