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Molecular insight into regioselectivity of transfructosylation catalyzed by GH68 levansucrase and beta-fructofuranosidase

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Title: Molecular insight into regioselectivity of transfructosylation catalyzed by GH68 levansucrase and beta-fructofuranosidase
Authors: Okuyama, Masayuki Browse this author →KAKEN DB
Serizawa, Ryo Browse this author
Tanuma, Masanari Browse this author
Kikuchi, Asako Browse this author
Sadahiro, Juri Browse this author
Tagami, Takayoshi Browse this author →KAKEN DB
Lang, Weeranuch Browse this author
Kimura, Atsuo Browse this author →KAKEN DB
Issue Date: Jan-2021
Publisher: Elsevier
Journal Title: Journal of Biological Chemistry (JBC)
Volume: 296
Start Page: 100398
Publisher DOI: 10.1016/j.jbc.2021.100398
Abstract: Glycoside hydrolase family 68 (GH68) enzymes catalyze beta-fructosyltransfer from sucrose to another sucrose, the so-called transfructosylation. Although regioselectivity of transfructosylation is divergent in GH68 enzymes, there is insufficient information available on the structural factor(s) involved in the selectivity. Here, we found two GH68 enzymes, beta-fructofuranosidase (FFZm) and levansucrase (LSZm), encoded tandemly in the genome of Zymomonas mobilis, displayed different selectivity: FFZm catalyzed the beta-(2 -> 1)-trans-fructosylation (1-TF), whereas LSZm did both of 1-TF and beta-(2 -> 6)-trans-fructosylation (6-TF). We identified His79(FFZm) and Ala343(FFZm) and their corresponding Asn84(LSZm) and Ser345(LSZm) respectively as the structural factors for those regioselectivities. LSZm with the respective substitution of FFZm-type His and Ala for its Asn84(LSZm) and Ser345(LSZm) (N84H/S345A-LSZm) lost 6-TF and enhanced 1-TF. Conversely, the LSZm-type replacement of His79(FFZm) and Ala343(FFZm) in FFZm (H79N/A343S-FFZm) almost lost 1-TF and acquired 6-TF. H79N/A343S-FFZm exhibited the selectivity like LSZm but did not produce the beta-(2 -> 6)-fructoside-linked levan and/or long levanooligo-saccharides that LSZm did. We assumed Phe189(LSZm) to be a responsible residue for the elongation of levan chain in LSZm and mutated the corresponding Leu187(FFZm) in FFZm to Phe. An H79N/L187F/A343S-FFZm produced a higher quantity of long levanooligosaccharides than H79N/A343S-FFZm (or H79N-FFZm), although without levan formation, suggesting that LSZm has another structural factor for levan production. We also found that FFZm generated a sucrose analog, beta-D-fructofuranosyl alpha-D-mannopyranoside, by beta-fructosyltransfer to D-mannose and regarded His79(FFZm) and Ala343(FFZm) as key residues for this acceptor specificity. In summary, this study provides insight into the structural factors of regioselectivity and acceptor specificity in transfructosylation of GH68 enzymes.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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