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Isolation and Characterization of Antimicrobial-Resistant Escherichia coli from Retail Meats from Roadside Butcheries in Uganda

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Title: Isolation and Characterization of Antimicrobial-Resistant Escherichia coli from Retail Meats from Roadside Butcheries in Uganda
Authors: Okubo, Torahiko Browse this author →KAKEN DB
Yossapol, Montira Browse this author
Ikushima, Shiori Browse this author
Kakooza, Steven Browse this author
Wampande, Eddie M. Browse this author
Asai, Tetsuo Browse this author →KAKEN DB
Tsuchida, Sayaka Browse this author →KAKEN DB
Ohya, Kenji Browse this author →KAKEN DB
Maruyama, Fumito Browse this author →KAKEN DB
Kabasa, John D. Browse this author
Ushida, Kazunari Browse this author →KAKEN DB
Keywords: retail meat
roadside butchery
E. coli
Issue Date: 6-Nov-2020
Publisher: Mary Ann Liebert
Journal Title: Foodborne Pathogens and Disease
Volume: 17
Issue: 11
Start Page: 666
End Page: 671
Publisher DOI: 10.1089/fpd.2020.2796
PMID: 32551973
Abstract: Retail meats are one of the main routes for spreading antimicrobial-resistant bacteria (ARB) from livestock to humans through the food chain. In African countries, retail meats are often sold at roadside butcheries without chilling or refrigeration. Retail meats in those butcheries are suspected to be contaminated by ARB, but it was not clear. In this study, we tested for the presence of antimicrobial-resistantEscherichia colifrom retail meats (n = 64) from roadside butcheries in Kampala, Uganda. The meat surfaces were swabbed and inoculated on PetriFilm SEC agar to isolateE. coli. We successfully isolatedE. colifrom 90.6% of these retail meat samples. We identified the phylogenetic type, antimicrobial susceptibility, and antimicrobial resistance genes prevalence between retail meat isolates (n = 89). Phylogenetic type B1 was identified from 70.8% of the retail meat isolates, suggesting that the isolates originated primarily from fecal contamination during meat processing. Tetracycline (TET)-resistant isolates withtetAand/ortetBgene(s) were the most frequently detected (28.1%), followed by ampicillin (AMP) resistance genes withbla(TEM)(15.7%,) and sulfamethoxazole-trimethoprim (SXT) resistance genes withsul2(15.7%). No extended-spectrum beta-lactamase-producing isolates were detected. A conjugation assay showed that resistance to AMP, TET, and SXT could be simultaneously transferred to recipients. These findings suggest that antimicrobial-resistantE. colican easily be transferred from farms to tables from retail meats obtained from roadside butcheries.
Rights: Final publication is available from Mary Ann Liebert, Inc., publishers
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大久保 寅彦

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