|
Hokkaido University Collection of Scholarly and Academic Papers >
Faculty of Pharmaceutical Sciences >
Peer-reviewed Journal Articles, etc >
Diverse intracellular signaling pathways mediate the effects of neurotensin on the excitability of type II neurons in the rat dorsolateral bed nucleus of the stria terminalis
| Title: | Diverse intracellular signaling pathways mediate the effects of neurotensin on the excitability of type II neurons in the rat dorsolateral bed nucleus of the stria terminalis |
| Authors: | Kaneko, Tomoyuki Browse this author | | Hara, Ryuto Browse this author | | Amano, Taiju Browse this author →KAKEN DB | | Minami, Masabumi Browse this author →KAKEN DB |
| Keywords: | Bed nucleus of the stria terminalis | | Ca2+/calmodulin-dependent protein | | kinase II | | Excitability | | Neurotensin | | Protein kinase A |
| Issue Date: | 5-Jun-2021 |
| Publisher: | Japanese Pharmacological Society |
| Journal Title: | Journal of pharmacological sciences |
| Volume: | 147 |
| Issue: | 1 |
| Start Page: | 86 |
| End Page: | 94 |
| Publisher DOI: | 10.1016/j.jphs.2021.05.013 |
| Abstract: | We examined the effects of neurotensin (NTS) on the excitability of type II neurons in the rat dorsolateral bed nucleus of the stria terminalis (dlBNST) using whole-cell patch-clamp electrophysiology. Bath-application of NTS depolarized type II dlBNST neurons. Analyses of the steady-state I-V relationships implied that the depolarizing effect of NTS is due to potassium conductance blocking. The depolarizing effect of NTS was abolished in the presence of a PLC inhibitor, but not affected by a protein kinase C inhibitor. In the presence of a CaMKII inhibitor, NTS showed depolarizing effects via the increase in nonselective cation conductance in addition to the decrease in potassium conductance. Unexpectedly, in the presence of a PKA inhibitor, NTS hyperpolarized type II dlBNST neurons. These results reveal that diverse signaling pathways mediate the effects of NTS on the excitability of type II dlBNST neurons. The elevation of intracellular Ca2+ levels via the inositol phosphate-mediated signaling activates both Ca2+-dependent adenylate cyclase (AC) and CaMKII. Activation of the AC-cAMP-PKA pathway exerts depolarizing effects on type II dlBNST neurons by decreasing potassium conductance and increasing non-selective cation conductance, whereas activation of the CaMKII pathway exerts hyperpolarizing effects on dlBNST neurons by decreasing non-selective cation conductance. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/83385 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
