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Laser-assisted biomineralization on human dentin for tooth surface functionalization
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
| Title: | Laser-assisted biomineralization on human dentin for tooth surface functionalization |
| Authors: | Oyane, Ayako Browse this author | | Saito, Noriyuki Browse this author | | Sakamaki, Ikuko Browse this author | | Koga, Kenji Browse this author | | Nakamura, Maki Browse this author | | Nathanael, A. Joseph Browse this author | | Yoshizawa, Noriko Browse this author | | Shitomi, Kanako Browse this author | | Mayumi, Kayoko Browse this author | | Miyaji, Hirofumi Browse this author →KAKEN DB |
| Keywords: | Hydroxyapatite | | Calcium phosphate (CaP) | | Dentin | | Laser | | Biomimetic process | | Coating |
| Issue Date: | Dec-2019 |
| Publisher: | Elsevier |
| Journal Title: | Materials science and engineering C : materials for biological applications |
| Volume: | 105 |
| Start Page: | 110061 |
| Publisher DOI: | 10.1016/j.msec.2019.110061 |
| PMID: | 31546361 |
| Abstract: | A technique for tooth surface modification with biocompatible calcium phosphate (CaP) has huge potential in dental applications. Recently, we achieved a facile and area-specific CaP coating on artificial materials by a laser-assisted biomimetic process (LAB process), which consists of pulsed laser irradiation in a supersaturated CaP solution. In this study, we induced the rapid biomineralization on the surface of human dentin by using the LAB process. A human dentin substrate was immersed in a supersaturated CaP solution, then its surface was irradiated with weak pulsed laser light for 30 min (LAB process). Ultrastructural analyses revealed that the pristine substrate had a demineralized collagenous layer on its surface due to the previous EDTA surface cleaning. After the LAB process, this collagenous layer disappeared and was replaced with a submicron-thick hydroxyapatite layer. We believe that the laser irradiation induced pseudo-biomineralization through the laser ablation of the collagenous layer, followed by CaP nucleation and growth at the dentin liquid interface. The mineralized layer on the dentin substrate consisted of needle-like hydroxyapatite nanocrystals, whose c-axes were weakly oriented along the direction perpendicular to the substrate surface. This LAB process would offer a new tool enabling tooth surface modification and functionalization through the in situ pseudo-biomineralization. |
| Rights: | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/83413 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮治 裕史
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