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Laser-assisted biomineralization on human dentin for tooth surface functionalization

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Title: Laser-assisted biomineralization on human dentin for tooth surface functionalization
Authors: Oyane, Ayako Browse this author
Saito, Noriyuki Browse this author
Sakamaki, Ikuko Browse this author
Koga, Kenji Browse this author
Nakamura, Maki Browse this author
Nathanael, A. Joseph Browse this author
Yoshizawa, Noriko Browse this author
Shitomi, Kanako Browse this author
Mayumi, Kayoko Browse this author
Miyaji, Hirofumi Browse this author →KAKEN DB
Keywords: Hydroxyapatite
Calcium phosphate (CaP)
Biomimetic process
Issue Date: Dec-2019
Publisher: Elsevier
Journal Title: Materials science and engineering C : materials for biological applications
Volume: 105
Start Page: 110061
Publisher DOI: 10.1016/j.msec.2019.110061
PMID: 31546361
Abstract: A technique for tooth surface modification with biocompatible calcium phosphate (CaP) has huge potential in dental applications. Recently, we achieved a facile and area-specific CaP coating on artificial materials by a laser-assisted biomimetic process (LAB process), which consists of pulsed laser irradiation in a supersaturated CaP solution. In this study, we induced the rapid biomineralization on the surface of human dentin by using the LAB process. A human dentin substrate was immersed in a supersaturated CaP solution, then its surface was irradiated with weak pulsed laser light for 30 min (LAB process). Ultrastructural analyses revealed that the pristine substrate had a demineralized collagenous layer on its surface due to the previous EDTA surface cleaning. After the LAB process, this collagenous layer disappeared and was replaced with a submicron-thick hydroxyapatite layer. We believe that the laser irradiation induced pseudo-biomineralization through the laser ablation of the collagenous layer, followed by CaP nucleation and growth at the dentin liquid interface. The mineralized layer on the dentin substrate consisted of needle-like hydroxyapatite nanocrystals, whose c-axes were weakly oriented along the direction perpendicular to the substrate surface. This LAB process would offer a new tool enabling tooth surface modification and functionalization through the in situ pseudo-biomineralization.
Rights: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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