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Unique Peripheral Antennas in the Photosystems of the Streptophyte Alga Mesostigma viride
| Title: | Unique Peripheral Antennas in the Photosystems of the Streptophyte Alga Mesostigma viride |
| Authors: | Aso, Michiki Browse this author | | Matsumae, Renon Browse this author | | Tanaka, Ayumi Browse this author →KAKEN DB | | Tanaka, Ryouichi Browse this author →KAKEN DB | | Takabayashi, Atsushi Browse this author →KAKEN DB |
| Keywords: | Green algae | | Iso-seq | | Mesostigma viride | | Native-PAGE | | Photosystem |
| Issue Date: | 8-Jan-2021 |
| Publisher: | Oxford University Press |
| Journal Title: | Plant and Cell Physiology |
| Volume: | 62 |
| Issue: | 3 |
| Start Page: | 436 |
| End Page: | 446 |
| Publisher DOI: | 10.1093/pcp/pcaa172 |
| Abstract: | Land plants evolved from a single group of streptophyte algae. One of the key factors needed for adaptation to a land environment is the modification in the peripheral antenna systems of photosystems (PSs). Here, the PSs of Mesostigma viride, one of the earliest-branching streptophyte algae, were analyzed to gain insight into their evolution. Isoform sequencing and phylogenetic analyses of light-harvesting complexes (LHCs) revealed that M. viride possesses three algae-specific LHCs, including algae-type LHCA2, LHCA9 and LHCP, while the streptophyte-specific LHCB6 was not identified. These data suggest that the acquisition of LHCB6 and the loss of algae-type LHCs occurred after the M. viride lineage branched off from other streptophytes. Clear-native (CN)-polyacrylamide gel electrophoresis (PAGE) resolved the photosynthetic complexes, including the PSI-PSII megacomplex, PSII-LHCII, two PSI-LHCI-LHCIIs, PSI-LHCI and the LHCII trimer. Results indicated that the higher-molecular weight PSI-LHCI-LHCII likely had more LHCII than the lower-molecular weight one, a unique feature of M. viride PSs. CN-PAGE coupled with mass spectrometry strongly suggested that the LHCP was bound to PSII-LHCII, while the algae-type LHCA2 and LHCA9 were bound to PSI-LHCI, both of which are different from those in land plants. Results of the present study strongly suggest that M. viride PSs possess unique features that were inherited from a common ancestor of streptophyte and chlorophyte algae. |
| Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in Plant and Cell Physiology following peer review. The version of record is available online at: https://academic.oup.com/pcp/article/62/3/436/6070176 , http://doi.org/10.1093/pcp/pcaa172. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/83775 |
| Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高林 厚史
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