HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >

Type XVII collagen interacts with the aPKC-PAR complex and maintains epidermal cell polarity

Files in This Item:
Watanabe_et_al_HUSCUP.pdf1.44 MBPDFView/Open
Watanabe_et_al_HUSCUP_Suppl. Fig.pdf473.17 kBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Type XVII collagen interacts with the aPKC-PAR complex and maintains epidermal cell polarity
Authors: Watanabe, Mika Browse this author
Kosumi, Hideyuki Browse this author
Osada, Shin-Ichi Browse this author →KAKEN DB
Takashima, Shota Browse this author
Wang, Yunan Browse this author
Nishie, Wataru Browse this author →KAKEN DB
Oikawa, Tsukasa Browse this author →KAKEN DB
Hirose, Tomonori Browse this author
Shimizu, Hiroshi Browse this author →KAKEN DB
Natsuga, Ken Browse this author →KAKEN DB
Keywords: atypical PKC
cell polarity
epidermal stem cell
Issue Date: Jan-2021
Publisher: John Wiley & Sons
Journal Title: Experimental dermatology
Volume: 30
Issue: 1
Start Page: 62
End Page: 67
Publisher DOI: 10.1111/exd.14196
Abstract: Type XVII collagen (COL17) is a transmembrane protein expressed in the basal epidermis. COL17 serves as a niche for epidermal stem cells, and although its reduction has been implicated in altering cell polarity and ageing of the epidermis, it is unknown how COL17 affects epidermal cell polarity. Here, we uncovered COL17 as a binding partner of the aPKC-PAR complex, which is a key regulating factor of cell polarity. Immunoprecipitation-immunoblot assay and protein-protein binding assay revealed that COL17 interacts with aPKC and PAR3. COL17 deficiency or epidermis-specific aPKC lambda deletion destabilized PAR3 distribution in the epidermis, while aPKC zeta knockout did not. Asymmetrical cell division was pronounced in COL17-null neonatal paw epidermis. These results show that COL17 is pivotal for maintaining epidermal cell polarity. Our study highlights the previously unrecognized role of COL17 in the basal keratinocytes.
Rights: This is the peer reviewed version of the following article: Watanabe, M, Kosumi, H, Osada, S‐I, et al. Type XVII collagen interacts with the aPKC‐PAR complex and maintains epidermal cell polarity. Exp Dermatol. 2021; 30: 62– 67., which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 夏賀 健

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University